Product Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
ICFC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?L per million cells in 100 ?L staining volume or 5 ?L per 100 ?L of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
- Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
Positive staining is stimulation and donor dependent. Upon stimulation at high concentrations of H202 some staining of B cells can be seen. Staining for ZAP70 Phospho (Tyr493) using clone A16043E can also be successfully performed using the True-Nuclear? Transcription Buffer Set and True-Phos? Perm Buffer.
- RRID
- AB_2894506 (BioLegend Cat. No. 396007) AB_2894506 (BioLegend Cat. No. 396008)
Antigen Details
- Cell Type
- NK cells, T cells
- Biology Area
- Cell Biology, Immunology, Signal Transduction
- Molecular Family
- Phospho-Proteins, Protein Kinases/Phosphatase, TCRs
- Gene ID
- 7535 View all products for this Gene ID
- UniProt
- View information about ZAP70 Phospho Tyr493 on UniProt.org
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