Product Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Recombinant human CCL2 (MCP-1)
- Formulation
- 0.2 ?m filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is < 0.01 EU/?g of the protein (< 0.001 ng/?g of the protein) as determined by the LAL test.
- Preparation
- The Ultra-LEAF? (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF? solution contains no preservative; handle under aseptic conditions.
- Application
-
Neut - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by neutralization of chemotaxis induced by recombinant human CCL2 (MCP-1) (Cat. No. 571406) at 0.1 ng/mL on mouse Baf3-mCCR2 transfectant chemoattractant cells. ND50 range: 40 - 300 ng/mL. It is recommended that the reagent be titrated for optimal performance for each application.
- RRID
- AB_2904453 (BioLegend Cat. No. 943803) AB_2904453 (BioLegend Cat. No. 943804)
Antigen Details
- Structure
- Chemokine
- Distribution
-
MCP-1 (CCL2) is secreted by fibroblast, endothelial cells, smooth muscle cells, tumor cells, and phytohemaglutinin stimulated mononuclear cells.
- Function
- MCP-1 induces migration of monocytes, memory/activated T cells, NK cells, myeloid dendritic cells, neutrophils, astrocytes, mesangial cells, and bone marrow endothelial cells. MCP-1 is induced by IL-1?, TNF, IFNγ, PDGF, IL-4, MIF, and IL-17.
- Interaction
- Monocytes, memory/activated T cells, NK cells, myeloid dendritic cells, neutrophils, astrocytes, mesangial cells, and bone marrow endothelial cells
- Ligand/Receptor
- MCP-1 mediates its cellular effects primarily through CCR2 receptor.
- Cell Type
- Astrocytes, Endothelial cells, Fibroblasts, Hematopoietic stem and progenitors, Monocytes
- Biology Area
- Cell Biology, Neurodegeneration, Neuroinflammation, Neuroscience, Stem Cells
- Molecular Family
- Cytokines/Chemokines
- Antigen References
-
- Loberg RD, et al. 2007. Cancer Research. 67:9417-9424.
- Gregory JL, et al. 2006. J Immunol. 177:8072-8079.
- Qui Z, et al. 2009. Immunology. 214:835-842.
- Reichel CA, et al. 2009. Arterioscler Thromb Vasc Biol. 109: 193268
- McQuibban GA, et al. 2002. Blood. 100:1160-1167
- Tesh GH, et al. 2008. Amm J Physiol Renal Physiol. 294:F697-F701.
- Gene ID
- 6347 View all products for this Gene ID
- UniProt
- View information about CCL2 on UniProt.org
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