Product Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Recombinant mouse CCL20 (MIP-3α)
- Formulation
- 0.2 ?m filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is < 0.01 EU/?g of the protein (< 0.001 ng/?g of the protein) as determined by the LAL test.
- Preparation
- The Ultra-LEAF? (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF? solution contains no preservative; handle under aseptic conditions.
- Application
-
Neut - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by neutralization of the chemotaxis induced by recombinant mouse CCL20 (MIP-3α) (Cat. No. 582306) at 0.3 ng/mL on mouse Baf3-hCCR6 transfectant chemoattractant cells. ND50 range: 0.08 - 0.4 ?g /mL. It is recommended that the reagent be titrated for optimal performance for each application.
- RRID
- AB_2894527 (BioLegend Cat. No. 942903) AB_2894527 (BioLegend Cat. No. 942904)
Antigen Details
- Structure
- Chemokine
- Distribution
-
Monocytes, T cells, neutrophils, endothelial cells, fibroblasts, keratinocytes, synoviocytes, DCs, pulmonary and intestinal epithelial cells, dermal microvascular endothelial cells
- Function
- Chemoattracts immature dendritic cells at sites of injury, upregulated by mediators of inflammation such as TNF or LPS, induced by Th17 cytokines (IL-17A, IL-22, and TNF-α) in keratinocyte.
- Interaction
- T cells, Th17, Tregs, B cells, DC subsets including CD11b+ CD8α-myeloid DCs, Langerhans cells (LC), CD34+ cell-derived immature myeloid DCs, and immature monocyte-derived DCs
- Ligand/Receptor
- CCR6 (CD196)
- Antigen References
-
- Varona R, et al. 1998. FEBS Lett. 440:188.
- Homey B, et al. 2000. J Immunol. 164:6621.
- Hoover DM, et al. 2002. J Biol Chem. 277:37647.
- Hirota K, et al. 2007. J Exp Med. 204:2803.
- Harper EG, et al. 2009. J Invest Dermatol. 129:2175.
- Ito T, et al. 2011. Exp Cell Res. 317:613.
- Gene ID
- 20297 View all products for this Gene ID
- UniProt
- View information about CCL20 on UniProt.org
我要詢(xún)價(jià)
*聯(lián)系方式：
(可以是QQ、MSN、電子郵箱、電話(huà)等，您的聯(lián)系方式不會(huì)被公開(kāi))
*內(nèi)容：
