Product Details
- Verified Reactivity
- Human, Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Human BTK peptide phosphorylated at Tyr 551.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
ICFC - Verified - Recommended Usage
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.125 - 2.0 ?g/ml (1:250-4000 dilution).?For intracellular flow cytometric staining, the suggested use of this reagent is ≤ 0.25 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
This clone was initially generated against a BTK peptide sequence containing phospho Tyrosine 551, but was shown to also bind to the highly homologous phospho Tyrosine 512 within the T-cell specific tyrosine kinase ITK. Due to complete conservation of these BTK and ITK phosphosites and the surrounding residues between human and mice, this antibody is predicted to recognize mouse BTK Phospho Tyrosine 551 and mouse ITK Phospho Tyrosine 517.
- RRID
- AB_2721728 (BioLegend Cat. No. 646901) AB_2721729 (BioLegend Cat. No. 646902)
Antigen Details
- Structure
- BTK encodes a 659 amino acid protein with a predicted molecular weight of 76.2 kD. Contains a PH domain (a.a. 1-133) that binds phosphatidylinositol (3,4,5)-triphosphate (PIP3) and polyphosphates, an SH3 domain (a.a. 214-274), an SH2 domain (a.a. 281-377), and a protein kinase domain (402-655).
ITK encodes a 620 amino acid protein with a predicted molecular weight of 71.8 kD. Contains one PH domain (a.a. 4-111) for plasma membrane recruitment, both SH3 (a.a. 171-231) and SH3 (a.a. 239-338) domains, and one protein kinase domain (a.a. 363-615). - Distribution
-
BTK: Cytosol, plasma membrane-associated, B cells
ITK: Cytosol, plasma membrane-associated, T cells - Function
- BTK: Links B cell receptor engagement with downstream signaling events
ITK: Links T cell receptor engagement with downstream signaling events - Interaction
- ARID3A, BLNK, CAV1, GNAQ, GTF2I, PLGC2, PRKD1, SH3BP5
- Ligand/Receptor
- PIP3
- Biology Area
- Cell Biology, Signal Transduction
- Molecular Family
- Phospho-Proteins, Protein Kinases/Phosphatase
- Antigen References
-
- Yang W, et al. 1997. Proc Natl Acad Sci U.S.A. 94:604.
- Park H, et al. 1996. Immunity. 4:515.
- Lin L, et al. 2009. Biochemistry. 48:2021.
- Heyeck SD, et al. 1997. J. Biol. Chem. 272:25401.
- Nore BF, et al. 2003. Biochim. Biophys. Acta. 1645:123.
- Gene ID
- 695 View all products for this Gene ID 3702 View all products for this Gene ID
- UniProt
- View information about BTK Phospho Tyr551/ITK Phospho Tyr512 on UniProt.org
