Product Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Recombinant mouse CXCL9
- Formulation
- 0.2 ?m filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is < 0.01 EU/?g of the protein (< 0.001 ng/?g of the protein) as determined by the LAL test.
- Preparation
- The Ultra-LEAF? (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF? solution contains no preservative; handle under aseptic conditions.
- Application
-
Neut - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by neutralization of the chemotaxis induced by recombinant mouse CXCL9 (MIG) (Cat. No. 578204) at 70 ng/mL on mouse Baf3-mCXCR3 transfectant chemoattractant cells. ND50 range: 0.15 - 1 ?g/mL. It is recommended that the reagent be titrated for optimal performance for each application.
- RRID
- AB_2892505 (BioLegend Cat. No. 942603) AB_2892505 (BioLegend Cat. No. 942604)
Antigen Details
- Structure
- Chemokine
- Distribution
-
CXCL9 is secreted by monocytes, macrophages, APC, eosinophils, endothelial cells, and B cells. Also, CXCL9 is secreted by fibroblast in inflammatory conditions.
- Function
- Induce chemotaxis, promote differentiation and multiplication of leukocytes, cause tissue extravasation, enhance CNS immunity; CXCL9/CXCR3 receptor regulates immune cell migration, differentiation, and activation
- Interaction
- Activated T lymphocytes, CD4 Th1, CD8+, NK, and NKT cells
- Ligand/Receptor
- CXCR3
- Antigen References
-
1. Loetscher M, et al. 1996. J. Exp. Med. 184:963.
2. Lambeir AM, et al. 2001. J. Biol. Chem. 276:29839.
3. Whiting D, et al. 2004. J. Immunol. 172:7417.
4. Gorbachev AV, et al. 2007. J. Immunol. 178:2278.
5. Rosenblum JM, et al. 2010. J. Immunol. 184:3450.
6. Crawford MA, et al. 2010. PLoS Pathog 6:e1001199. - Gene ID
- 17329 View all products for this Gene ID
- UniProt
- View information about CXCL9 on UniProt.org
