Product Details
- Verified Reactivity
- Human, Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Synthetic human STAT3 peptide phosphorylated at serine 727
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
ICC - Verified - Recommended Usage
Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 1.0?- 2.0??g/mL.?For immunocytochemistry, a concentration range of 1.0?- 5.0?μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Clones A16089A and A16089B are both specific for STAT3 phosphorylated at serine 727, but performed differently for different applications tested:
Both clones displayed strong specificity and selectivity for WB, but clone A16089A displayed a considerably stronger affinity for the target compared to A16089B
Both clones performed well for ICC, though clone A16089A displayed mild extranuclear staining
A16089B performed well for ChIP, A16089A failed ChIP application testing- RRID
- AB_2892483 (BioLegend Cat. No. 669501) AB_2892483 (BioLegend Cat. No. 669502)
Antigen Details
- Structure
- STAT3 is a 770 amino acid protein with a predicted molecular weight of 88 kDa. It consists of a DNA binding domain, a SH2 domain, a regulatory tyrosine responsible for binding of SH2 domain, and a C-terminal transactivation domain.
- Distribution
-
Cytosol and nucleus/Ubiquitously expressed
- Function
- Transcription factor
- Antigen References
-
- Akira S, et al. 1994. Cell. 77:63-71.
- Zhang X, et al. 1995. Science. 267:1990-4.
- Sanchez-Margalet V & Martin-Romero C. 2001. Cell Immunol. 211:30-6.
- Simon AR, et al. 2000. Science. 290:144-7.
- Hoey T & Grusby MJ. 1999. Adv Immunol. 71:145-62.
- Gene ID
- 6774 View all products for this Gene ID
- UniProt
- View information about STAT3 Phospho Ser727 on UniProt.org
