Product Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Human Stat3 peptide phosphorylated at Ser 727. Complete Freund's adjuvant.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
ICFC - Quality tested
ICC, ChIP - Verified - Recommended Usage
Each lot of this antibody is quality control tested by intracellular flow cytometry using our?True-Phos? Perm Buffer in Cell Suspensions Protocol. For flow cytometric staining, the suggested use of this reagent is ≤ 0.03 ?g per million cells in 100 ?l volume.?For immunocytochemistry, a concentration of 2.5 μg/mL is recommended. For ChIP applications, the suggested dilution is 1:50-1:100 by volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
The STAT3 Phospho (Ser727) antibody recognizes the regulatory serine phosphorylation of human STAT3 protein.
- RRID
- AB_2728515 (BioLegend Cat. No. 698901) AB_2728516 (BioLegend Cat. No. 698902)
Antigen Details
- Structure
- STAT3 is a 770 amino acid protein of 88 kD. It consists of a DNA binding domain, a SH2 domain, a regulatory tyrosine responsible for binding of SH2 domain, and a C-terminal transactivation domain.
- Distribution
-
Ubiquitous
- Function
- STAT3 is also involved in gp130 signaling and binds to IL-6 response elements in various acute phase protein promoters. STAT3 is phosphorylated by signaling from IFNs, EGF, FGF, IL-5, HGF, LIF, and BMP2. STAT3 activity is inhibited by PIAS3 and GRIM-19 and can also be regulated by the Rac1 protein.
- Interaction
- When activated, STAT3 dimerizes and translocates to the nucleus where it regulates gene expression.
- Biology Area
- Cell Biology, Neuroscience, Neuroscience Cell Markers, Signal Transduction, Transcription Factors
- Molecular Family
- Nuclear Markers, Phospho-Proteins
- Antigen References
-
1. Akira S, et al. 1994. Cell. 77:63.
2. Zhang X, et al. 1995. Science 267:1990.
3. Sanchez-Margalet V, et al. 2001. Cell. Immunol. 211:30.
4. Simon A, et al. 2000. Science 290:144.
5. Hoey T, et al. 1999. Adv. Immunol. 71:145. - Gene ID
- 6774 View all products for this Gene ID
- UniProt
- View information about STAT3 Phospho Ser727 on UniProt.org
