Product Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Recombinant human BMP-14 (GDF-5)
- Formulation
- 0.2 ?m filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is < 0.01 EU/?g of the protein (< 0.001 ng/?g of the protein) as determined by the LAL test.
- Preparation
- The Ultra-LEAF? (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF? solution contains no preservative; handle under aseptic conditions.
- Application
-
Neut - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by neutralizing the differentiation induced by recombinant human BMP-14 (GDF-5) (Cat. No. 779506) at 1.5 ?g/mL on ATDC5 mouse chondrogenic cells. ND50 range: 3?– 15??g/mL. It is recommended that the reagent be titrated for optimal performance for each application.
- RRID
- AB_2910514 (BioLegend Cat. No. 949203) AB_2910514 (BioLegend Cat. No. 949204)
Antigen Details
- Structure
- Homodimer
- Distribution
-
Predominantly expressed in cartilaginous tissues, precartilaginous mesenchymal condensation, and throughout the cartilaginous cores of the developing long bones
- Function
- Skeletal development during embryogenesis, joint formation, tendon repair, and odontogenesis. MicroRNA-21 suppresses the expression of GDF-5 by inducing GDF-5 mRNA decay in chondrocytes. Noggin inhibits GDF-5.
- Interaction
- Chondrocytes, osteoblast, periosteum cells, fibroblasts, hippocampal pyramidal cells, and dopaminergic midbrain neurons
- Ligand/Receptor
- BMPR1b, BMPRII, and ACTRIIA
- Cell Type
- Embryonic Stem Cells, Fibroblasts, Hematopoietic stem and progenitors, Mesenchymal cells, Mesenchymal Stem Cells, Neural Stem Cells, Neurons
- Biology Area
- Cell Biology, Neuroscience, Stem Cells, Synaptic Biology
- Molecular Family
- Cytokines/Chemokines, Growth Factors
- Antigen References
-
- Chang SC, et al. 1994. J Biol Chem. 269:28227-34.
- Hotten G, et al. 1994. Biochem Biophys Res Commun. 204:646-52.
- Nishitoh H, et al. 1996. J Biol Chem. 271:21345-52.
- Groppe J, et al. 2002. Nature. 420:636-42.
- Chen X, et al. 2006. J Mol Cell Cardiol. 41:26-33.
- Kleinschmidt K, et al. 2013. Biomaterials. 34:5926-36.
- Osorio C, et al. 2013. Development. 140:4751-62.
- Zhang Y, et al. 2014. Exp Mol Med. 46:e79.
- Li YF, et al. 2015. Int J Clin. Exp Pathol. 8:719-26.
- Gene ID
- 8200 View all products for this Gene ID
- UniProt
- View information about BMP-14 on UniProt.org
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