Product Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Recombinant Human DJ-1
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
Direct ELISA, IHC-P - Verified - Recommended Usage
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 5.0 ?g per ml. For Direct ELISA, a concentration range of 0.002 - 1.0 ?g/mL is recommended. For immunohistochemistry, a concentration range of 1.0 - 5.0 ?g/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
- RRID
- AB_2721732 (BioLegend Cat. No. 851501) AB_2721733 (BioLegend Cat. No. 851502)
Antigen Details
- Structure
- Human DJ-1 is a 189 amino acid protein with an apparent molecular weight of 20 kD. The protein consists of 7 β-strands and 7 α-helices, and exits as a dimer.
- Distribution
-
Tissue distribution: Highly expressed in pancreas, kidney, skeletal muscle, liver, testis and heart. Detected at slightly lower levels in brain and placenta.
Cellular distribution: Mitochondria, nucleus, cytosol, plasma membrane, and extracellular. - Function
- DJ-1 functions as an oxidative stress sensor and redox-sensitive chaperone.
- Interaction
- PINK1, Parkin, EFCAB6/DJBP, OTUD7B, BBS1, HIPK1, CLCF1 and MTERF.
- Biology Area
- Cell Biology, Mitochondrial Function, Neurodegeneration, Neuroinflammation, Neuroscience, Neuroscience Cell Markers, Protein Misfolding and Aggregation, Protein Trafficking and Clearance
- Molecular Family
- Mitochondrial Markers
- Antigen References
-
1. Saito Y. 2014. J. Clin. Biochem. Nutr. 54(3):138-44. PubMed
2. Ariga H, et al. 2013. Oxid. Med. Cell Longev. 2013:683920. PubMed
3. Bonifati V, et al. 2003. Science (5604): 256–9. PubMed - Gene ID
- 11315 View all products for this Gene ID
- UniProt
- View information about DJ-1 on UniProt.org
