Product Details
- Verified Reactivity
- Human, Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Human BTK peptide phosphorylated at Tyr 223, Complete Freund's adjuvant.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
ICFC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by intracellular flow cytometry using our?Intracellular Flow Cytometry Staining Protocol. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.
- Excitation Laser
- Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
A16128B was generated in the same immunization that produced clone A16128C (anti-BTK Phospho (Tyr223)/ITK Phospho (Tyr180)). Both clones bind BTK Phospho Tyr223 with similar affinity, but in comparison to clone A16128C, clone A16128B has weaker recognition of phosphorylated Tyrosine 180 of ITK by intracellular staining and flow cytometric analysis.
- Product Citations
-
- RRID
- AB_2721620 (BioLegend Cat. No. 601703) AB_2721621 (BioLegend Cat. No. 601704)
Antigen Details
- Structure
- BTK encodes a 659 amino acid protein with a predicted molecular weight of 76.2 kD. Contains a PH domain (a.a. 1-133) that binds phosphatidylinositol (3,4,5)-triphosphate (PIP3) and polyphosphates, an SH3 domain (a.a. 214-274), an SH2 domain (a.a. 281-377), and a protein kinase domain (402-655).
- Distribution
-
Cytosol, plasma-membrane associated
- Function
- Links B cell receptor engagement with downstream signaling events
- Interaction
- ARID3A, BLNK, CAV1, GNAQ, GTF2I, PLGC2, PRKD1, SH3BP5
- Ligand/Receptor
- PIP3
- Biology Area
- Cell Biology, Signal Transduction
- Molecular Family
- Phospho-Proteins, Protein Kinases/Phosphatase
- Antigen References
-
- Krysiak K, et al. 2017. Blood. 129:473.
- Burger JA, et al. 2016. Nat Commun. 7:11589.
- Wang Q, et al. 2015. Elife. 4:e06074.
- Ito M, et al. 2015. Nat. Commun. 6:7360.
- Pillinger G, et al. 2015. Sci. Rep. 5:12949.
- Cinar M, et al. 2013. Leuk. Res. 37:1271.
- Doyle SL, et al. 2007 J. Biol. Chem. 282:36953.
- Nore BF, et al. 2003. Biochem. Biophys. Acta. 1645:123.
- Li Z, et al. 1997. Proc. Natl. Acad. Sci. 94:13820.
- Gene ID
- 695 View all products for this Gene ID
- UniProt
- View information about BTK Phospho Tyr223 on UniProt.org
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