Product Details
- Verified Reactivity
- Human, Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Human BTK peptide phosphorylated at Tyr 223, Complete Freund's adjuvant.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
ICFC - Verified - Recommended Usage
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 2.0 ?g per ml (1:500-1:250). For flow cytometric staining, the suggested use of this reagent is 0.5 ?g per million cells in 100 ?l volume.?It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
A16128B was generated in the same immunization that produced clone A16128C (anti-BTK Phospho (Tyr223)/ITK Phospho (Tyr180)). Both clones bind BTK Phospho Tyr223 with similar affinity, but in comparison to clone A16128C, clone A16128B has weaker recognition of phosphorylated Tyrosine 180 of ITK by intracellular staining and flow cytometric analysis.
- RRID
- AB_2715906 (BioLegend Cat. No. 601701) AB_2715907 (BioLegend Cat. No. 601702)
Antigen Details
- Structure
- BTK encodes a 659 amino acid protein with a predicted molecular weight of 76.2 kD. Contains a PH domain (a.a. 1-133) that binds phosphatidylinositol (3,4,5)-triphosphate (PIP3) and polyphosphates, an SH3 domain (a.a. 214-274), an SH2 domain (a.a. 281-377), and a protein kinase domain (402-655).
- Distribution
-
Cytosol, plasma-membrane associated
- Function
- Links B cell receptor engagement with downstream signaling events
- Interaction
- ARID3A, BLNK, CAV1, GNAQ, GTF2I, PLGC2, PRKD1, SH3BP5
- Ligand/Receptor
- PIP3
- Biology Area
- Cell Biology, Signal Transduction
- Molecular Family
- Phospho-Proteins, Protein Kinases/Phosphatase
- Antigen References
-
- Krysiak K, et al. 2017. Blood. 129:473.
- Burger JA, et al. 2016. Nat Commun. 7:11589.
- Wang Q, et al. 2015. Elife. 4:e06074.
- Ito M, et al. 2015. Nat. Commun. 6:7360.
- Pillinger G, et al. 2015. Sci. Rep. 5:12949.
- Cinar M, et al. 2013. Leuk. Res. 37:1271.
- Doyle SL, et al. 2007 J. Biol. Chem. 282:36953.
- Nore BF, et al. 2003. Biochem. Biophys. Acta. 1645:123.
- Li Z, et al. 1997. Proc. Natl. Acad. Sci. 94:13820.
- Gene ID
- 695 View all products for this Gene ID
- UniProt
- View information about BTK Phospho Tyr223 on UniProt.org
