Product Details
- Verified Reactivity
- Human, Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Human BTK peptide phosphorylated at Tyr 223, Complete Freund's adjuvant.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
ICFC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Phos? Perm Buffer in Cell Suspensions Protocol. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.
- Excitation Laser
- Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
Clones A16128C and A16128B (anti-BTK Phospho (Tyr223)) both bind BTK Phospho Tyr223 with similar affinity, but in comparison to clone A16128B, clone A16128C has a much stronger recognition of phosphorylated Tyrosine 180 of ITK by intracellular staining and flow cytometric analysis. ITK is the T cell-specific homolog of BTK that is not found in B cells. Similarly, T cells lack BTK1.
- Application References
(PubMed link indicates BioLegend citation) -
- Tomlinson MG, et al. 2004. Mol. Cell. Biol. 24: 2455
- RRID
- AB_2721622 (BioLegend Cat. No. 605203) AB_2721623 (BioLegend Cat. No. 605204)
Antigen Details
- Structure
- BTK encodes a 659 amino acid protein with a predicted molecular weight of 76.2 kD. Contains a PH domain (a.a. 1-133) that binds phosphatidylinositol (3,4,5)-triphosphate (PIP3) and polyphosphates, an SH3 domain (a.a. 214-274), an SH2 domain (a.a. 281-377), and a protein kinase domain (402-655).
- Distribution
-
Cytosol, plasma-membrane associated, B cells
- Function
- Links B cell receptor engagement with downstream signaling events
- Interaction
- ARID3A, BLNK, CAV1, GNAQ, GTF2I, PLGC2, PRKD1, SH3BP5
- Ligand/Receptor
- PIP3
- Biology Area
- Cell Biology, Signal Transduction
- Molecular Family
- Phospho-Proteins
- Antigen References
-
1. Krysiak K, et al. 2017. Blood. 129:473.
2. Burger JA, et al. 2016. Nat Commun. 7:11589.
3. Wang Q, et al. 2015. Elife. 4:e06074.
4. Ito M, et al. 2015. Nat. Commun. 6:7360.
5. Pillinger G, et al. 2015. Sci. Rep. 5:12949.
6. Cinar M, et al. 2013. Leuk. Res. 37:1271.
7. Doyle SL, et al. 2007 J. Biol. Chem. 282:36953.
8. Nore BF, et al. 2003. Biochem. Biophys. Acta. 1645:123.
9. Li Z, et al. 1997. Proc. Natl. Acad. Sci. 94:13820. - Gene ID
- 695 View all products for this Gene ID
- UniProt
- View information about BTK Phospho Tyr223/ITK Phospho Tyr180 on UniProt.org
