Product Details
- Verified Reactivity
- Human, Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Human BTK peptide phosphorylated at Tyr 223, Complete Freund's adjuvant.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
ICFC - Verified - Recommended Usage
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is?2.0 ug/mL (1:250 dilution).?For?intracellular flow cytometric staining, the suggested use of this reagent is 0.25 ?g per million cells in 100 ?l volume.?It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Clones A16128C and A16128B (anti-BTK Phospho (Tyr223)) both bind BTK Phospho Tyr223 with similar affinity, but in comparison to clone A16128B, clone A16128C has a much stronger recognition of phosphorylated Tyrosine 180 of ITK by intracellular staining and flow cytometric analysis. ITK is the T cell-specific homolog of BTK that is not found in B cells. Similarly, T cells lack BTK1.
- Application References
(PubMed link indicates BioLegend citation) -
- Tomlinson MG, et al. 2004. Mol. Cell. Biol. 24: 2455
- RRID
- AB_2721726 (BioLegend Cat. No. 605201) AB_2721727 (BioLegend Cat. No. 605202)
Antigen Details
- Structure
- BTK encodes a 659 amino acid protein with a predicted molecular weight of 76.2 kD. Contains a PH domain (a.a. 1-133) that binds phosphatidylinositol (3,4,5)-triphosphate (PIP3) and polyphosphates, an SH3 domain (a.a. 214-274), an SH2 domain (a.a. 281-377), and a protein kinase domain (402-655).
- Distribution
-
Cytosol, plasma-membrane associated, B cells
- Function
- Links B cell receptor engagement with downstream signaling events
- Interaction
- ARID3A, BLNK, CAV1, GNAQ, GTF2I, PLGC2, PRKD1, SH3BP5
- Ligand/Receptor
- PIP3
- Biology Area
- Cell Biology, Signal Transduction
- Molecular Family
- Phospho-Proteins
- Antigen References
-
1. Krysiak K, et al. 2017. Blood. 129:473.
2. Burger JA, et al. 2016. Nat Commun. 7:11589.
3. Wang Q, et al. 2015. Elife. 4:e06074.
4. Ito M, et al. 2015. Nat. Commun. 6:7360.
5. Pillinger G, et al. 2015. Sci. Rep. 5:12949.
6. Cinar M, et al. 2013. Leuk. Res. 37:1271.
7. Doyle SL, et al. 2007 J. Biol. Chem. 282:36953.
8. Nore BF, et al. 2003. Biochem. Biophys. Acta. 1645:123.
9. Li Z, et al. 1997. Proc. Natl. Acad. Sci. 94:13820. - Gene ID
- 695 View all products for this Gene ID
- UniProt
- View information about BTK Phospho Tyr223/ITK Phospho Tyr180 on UniProt.org
