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PE/Cyanine7 anti-human CD4 Antibody

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Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Human CD4 T cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Additional Product Notes
BioLegend is in the process of converting the name PE/Cy7 to PE/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our PE/Cyanine7 products. Please contact Technical Service if you have any questions.
Product Citations
  1. Ahmed R et al. 2019. Cell. 177(6):1583-1599 . PubMed
  2. Minns D, et al. 2021. Front Immunol. 12:633486. PubMed
  3. Hearnden R, et al. 2021. STAR Protocols. 2(2):100422. PubMed
  4. Wang K, et al. 2020. Signal Transduct Target Ther. 0.404861111. PubMed
  5. Yang W, et al. 2020. Nat Commun. 3.553472222. PubMed
  6. Kramer KJ, et al. 2022. Nat Commun. 13:3466. PubMed
  7. Park JA, et al. 2021. Journal for ImmunoTherapy of Cancer. 9(5):. PubMed
  8. Wei JL, et al. 2021. J Immunother Cancer. 9: . PubMed
  9. Mu Z, et al. 2021. Genome Biol. 122:22. PubMed
  10. Luo Y, et al. 2021. Nat Commun. 12:3913. PubMed
  11. Su X, et al. 2022. J Transl Med. 20:378. PubMed
  12. Faust HJ, et al. 2020. J Clin Invest. 130:5493. PubMed
  13. Dai Z, et al. 2022. Signal Transduct Target Ther. 7:85. PubMed
  14. Le J, et al. 2020. Immunity. 52(6):1105-1118.e9. PubMed
  15. Park JA, et al. 2020. J Hematol Oncol. 0.661111111. PubMed
  16. Egedal JH, et al. 2021. Mucosal Immunol. 14:1203. PubMed
  17. ?yszkiewicz M, et al. 2020. Nat Commun. 11:1031. PubMed
  18. Pekayvaz K, et al. 2022. Nat Commun. 13:1018. PubMed
  19. Corrado M, et al. 2020. Cell Metab. 32:981. PubMed
RRID
AB_2565661 (BioLegend Cat. No. 357409) AB_2565662 (BioLegend Cat. No. 357410)

Antigen Details

Structure
55 kD, type I transmembrane glycoprotein, member of the Ig superfamily
Distribution

Helper T cells, subset of thymocytes, monocytes/macrophages, type II NKT cells

Interaction
Part of the TCR/CD3 complex
Ligand/Receptor
β2 domain from the MHC class II
Cell Type
T cells, Monocytes, Macrophages, NKT cells
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Zhu J, et al. 2010. Annu Rev. Immunol. 28:445.
2. Vignali DA. 2010. J. Immunol. 184:5933.
3. Zhou L, et al. 2009. Immunity 30:646.
4. Singer A, et al. 2008. Nat. Rev. Immunol. 8:788.
5. Zhu J and Paul WE. 2008. Blood 112:1557.

Gene ID
920 View all products for this Gene ID
UniProt
View information about CD4 on UniProt.org
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