Product Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
ChIP - Quality tested
WB, ICFC, ICC - Verified - Recommended Usage
Each lot of this antibody is quality control tested by ChIP Assay. The suggested dilution for ChIP application is 1:50 by volume.?For Western blotting, the suggested use of this reagent is 0.1 - 2.0 ?g per ml.?For?intracellular flow cytometric staining, the suggested use of this reagent is ≤ 0.5 ?g per million cells in 100 ?l volume.?For immunocytochemistry, a concentration of 5.0 μg/ml is recommended. It is recommended that the reagent to be titrated for optimal performance before each experiment.
For intracellular flow cytometry our True-Nuclear? Transcription Factor Staining Protocol is recommended.- Application Notes
Clone A17012A recognizes STAT1 phosphorylated at Tyrosine 701 (Tyr701).
When using this clone for ICC, we recommend using methanol to permeabilize fixed cells.- RRID
- AB_2728501 (BioLegend Cat. No. 666405) AB_2728502 (BioLegend Cat. No. 666406)
Antigen Details
- Structure
- Two isoforms of STAT1 exist as a result of alternative splicing. Isoform α is a 750 amino acid protein with a predicted molecular weight of 87 kD; the β isoform is a 712 amino acid protein with a predicted molecular weight of 83 kD.
- Distribution
-
Ubiquitous tissue expression, nucleoplasmic-cytosolic distribution
- Function
- Immune response activation, cell signaling
- Interaction
- STAT1, STAT2, IRF9, ERBB4, JAK1, JAK2, TYK2, TNK1, SHP2
- Biology Area
- Cell Biology
- Molecular Family
- Nuclear Markers, Phospho-Proteins
- Antigen References
-
- Moretti S, et al. 2017. J. Biol. Chem. 292: 1785.
- Wei J, et al. 2015. J. Immunol. 195: 2870.
- Sung PS, et al. 2015. Proc. Natl. Acad. Sci. USA. 112: 10443
- Ooi EL, et al. 2014. Proc. Natl. Acad. Sci. USA. 111: 1909.
- Wu TR, et al. 2002. J. Biol. Chem. 277: 47572.
- Horvath, et al. 1996. J. Virol. 70: 647.
- Haque SJ, et al. 1995. J. Biol. Chem. 270: 25709.
- Gene ID
- 6772 View all products for this Gene ID
- UniProt
- View information about STAT1 Phospho Tyr701 on UniProt.org
