Product Details
- Verified Reactivity
- Human, Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Human Lck peptide phosphorylated around Tyr505.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
ICC, IP - Verified - Recommended Usage
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.25 - 2.0 ug/mL (1:250-2000 dilution) ?g per ml. For immunocytochemistry, a concentration range of 1.0 - 2.0 μg/ml (1:100-1:250 dilution) is recommended.?For immunoprecipitation, the suggested use of this reagent is 2.5 ?g per ml. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Clone A17013D was derived from a human Lck peptide phosphorylated at Y505 immunogen, but still displayed pan reactivity towards the unmodified peptide during western blot screening. This clone was moderately stronger than BioLegend clone LCK-01 for western blot and substantially stronger for immunofluorescence microscopy in parallel testing. But it shows a weak affinity towards mouse Lck. This clone is predicted to recognize both 58 and 61 kD isoforms based off the presence of the immunizing epitope in both proteins and may also recognize certain SRC tyrosine kinase family members due to high sequence similarity.
Phosphorylation of Lck Y505 reduces binding of this antibody by western blot (please see Fig. 3); we recommend LCK-01 as a pan control antibody.
The additional band of ~ 50 kD detected bellow the canonical Lck band in IP experiment could be Lck degradation product.- RRID
- AB_2728456 (BioLegend Cat. No. 601001) AB_2728457 (BioLegend Cat. No. 601002)
Antigen Details
- Distribution
-
Cytoplasmic protein, bound to cytoplasmic CD4 and CD8 in T lymphocytes.
- Function
- Participates in antigen-induced T cell activation, phosphorylates zeta chain of T cell receptor. Involved in interleukin-2 induced onset of cell cycle progression.
- Interaction
- Associates with CD4 and CD8 co-receptors. Binds to PI3K through SH3 interactions, binds to tyrosine phosphorylated KHDRBS1/p70 through SH2 domain. Interacts with SAP transmembrane tyrosine phosphatase.
- Biology Area
- Cell Biology, Signal Transduction, Transcription Factors
- Antigen References
-
- Koga Y, et al. 1986. Eur. J. Immunol. 16:1643.
- Vogel LB, et al. 1995. Biochem. Biophys. Acta 1264:168.
- Vogel LB and Fujita DJ. 1993. Mol. Cell. Biol. 13:7408.
- Bergman M, et al. 1992. EMBO J. 11:2919.
- Gene ID
- 3932 View all products for this Gene ID
- UniProt
- View information about Lck on UniProt.org
