Product Details
- Verified Reactivity
- Human, Mouse?
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Phosphorylated human STAT5A at Tyr694
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
ChIP - Quality tested
ICFC - Verified - Recommended Usage
Each lot of this antibody is quality control tested by ChIP Assay. The suggested dilution for ChIP application is 1:50 - 1:200 by volume. For flow cytometric staining, the suggested use of this reagent is ≤0.125 ?g per million cells in 100 ?l volume. It is recommended that the reagent to be titrated for optimal performance before each experiment.
- Application Notes
Does not cross-react with mouse for ICFC.
?Based on in-house testing, clone A17016B cross-reacts to Mouse STAT5 Phospho (Tyr694) in Western blots, but it is not recommended for intracellular flow cytometry application due to dim staining- RRID
- AB_2734545 (BioLegend Cat. No. 699603) AB_2734546 (BioLegend Cat. No. 699604)
Antigen Details
- Structure
- 794 amino acids, predicted molecular weight of 91 kD; contains a SH2 domains responsible for homodimerization or heterodimerization.
- Distribution
-
Cytoplasm; phosphorylated form translocates to the nucleus.
- Function
- STAT5 is a transcription regulator and is involved in cell differentiation, proliferation, and survival, in response to several cytokines, such as interleukins, erythropoietin, growth hormone, thrombopoietin, and prolactin.
- Interaction
- Forms a homodimer or a heterodimer with other family members. Interacts with NCOA1, SOCS7, CBP, p300, SMRT and ERBB4.
- Cell Type
- B cells, Th17, Tregs
- Biology Area
- Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology, Signal Transduction, Transcription Factors
- Molecular Family
- Nuclear Markers, Phospho-Proteins
- Antigen References
-
1. Bell BD, et al. 2013. Nat. Immunol. 14:364.
2. Cholez E, et al. 2012. Leukemia. 26:2390.
3. Heltemes-Harris LM, et al. 2012. Curr. Opin. Immunol. 24:146.
4. Heltemes-Harris LM, et al. 2011. Ann. N Y Acad. Sci. 1217:18.
5. Malin S, et al. 2010. Curr. Opin. Immunol. 22:168.
6. Xu J, et al. 2009. J. Neurosci. 29:2022. - Gene ID
- 6776 View all products for this Gene ID
- UniProt
- View information about STAT5 Phospho on UniProt.org
