Product Details
- Verified Reactivity
- Human, Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Synthetic peptide from human RPS6 phosphorylated at Serines 235 and 236
- Formulation
- This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
- Storage & Handling
- Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.
- Application
-
WB - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested dilution?of this reagent is 1:500?- 1:2000. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Due to complete conservation of the immunizing sequence between humans, mouse and rat, this clone is is predicted to react with rat RPS6 phosphorylated at serines 235 and 236.
- RRID
- AB_2910485 (BioLegend Cat. No. 608611) AB_2910485 (BioLegend Cat. No. 608612)
Antigen Details
- Structure
- RPS6 is a 249 amino acid protein with a predicted molecular weight of 28.6 kD.
- Distribution
-
Cytosol, nucleus, ER, ubiquitous expression.
- Function
- Phosphorylation events at a cluster of serine residues (Ser 235, 236, 240, and 244) at the carboxy-terminus of RPS6 results in increased global translational efficiency. These sites are dephosphorylated during growth arrest.
- Interaction
- RPS6AK1 and RPS6AK3; other subunits of 40S ribosomal complex.
- Biology Area
- Cell Biology, Cell Proliferation and Viability, Protein Synthesis, Signal Transduction
- Molecular Family
- Nuclear Markers, Phospho-Proteins
- Antigen References
-
- Jefferies HB, et al. 1997. EMBO J. 16:3693.
- Ruvinsky I, et al. 2005. Genes. Dev.19:2199.
- Chumacher AM, et al. 2006. Biochemistry. 45:13614
- Roux PP, et al. 2007. J. Biol. Chem. 282:14056.
- Stevens C, et al. 2009. J. Biol. Chem. 284:334.
- Schlafli P, et al. 2011. FEBS J. 278:1757.
- Gene ID
- 6194 View all products for this Gene ID
- UniProt
- View information about RPS6 Phospho on UniProt.org
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