Product Details
- Verified Reactivity
- Human, Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
ICC - Verified - Recommended Usage
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 1.0 ?g per ml. For immunocytochemistry, a concentration of 5 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Based off complete sequence homology of the immunizing peptide, this clone is predicted to recognize both mouse and rat mTOR phosphorylated at serine 2448.
NIH/3T3 cells (murine origin) used in this experiment contain less mTOR than the human-derived HeLa cells (see pan mTOR blot). Thus, the reduced signal observed in these lysates is due to less total mTOR and not a decreased affinity of this clone for mouse mTOR phosphor (Ser2448).- Product Citations
-
- RRID
- AB_2801105 (BioLegend Cat. No. 610301) AB_2801106 (BioLegend Cat. No. 610302)
Antigen Details
- Structure
- mTOR is a 2,549 amino acid protein with a predicted molecular weight of ~289 kD.
- Distribution
-
Ubiquitously expressed; cytsosolic, lysosomal, and nuclear cellular localization
- Function
- Regulates cell proliferation and metabolism
- Interaction
- Part of the mammalian target of rapamycin complex 1 (mTORC1) which contains MTOR, MLST8, RPTOR, AKT1S1/PRAS40, and DEPTOR; also part of the mammalian target of rapamycin complex 2 (mTORC2) which contains MTOR, MLST8, PRR5, RICTOR, MAPKAP1, and DEPTOR.
- Biology Area
- Cell Biology, Cell Motility/Cytoskeleton/Structure, Cell Proliferation and Viability, Protein Synthesis, Signal Transduction
- Molecular Family
- Phospho-Proteins, Protein Kinases/Phosphatase
- Antigen References
-
- Brown EJ, et al. 1994. Nature. 369:756-8.
- Dennis P, et al. 2001. Science. 294:1102.
- Fingar D, et al. 2002. Genes Dev. 16:1472.
- Nojima H, et al. 2003. J. Biol. Chem. 278:15461.
- Gene ID
- 2475 View all products for this Gene ID
- UniProt
- View information about mTOR Phospho Ser2448 on UniProt.org
