Product Details
- Verified Reactivity
- Human, Rat
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Purified recombinant human EIF2AK2
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody was purified by affinity chromatography.
- Application
-
WB - Quality tested
ICC, IHC-P - Verified - Recommended Usage
Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.5 - 1.0 ?g/mL. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 0.5 - 10 ?g/mL is suggested. For immunocytochemistry, a concentration range of 0.5 - 10 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
This antibody reacts stronger with rat tissue samples than human cell line samples. Recommended WB usage for rat brain: 0.5 - 2 ?g/mL; Recommended WB usage for human cell lines: 2 - 10 ?g/mL
- RRID
- AB_2832878 (BioLegend Cat. No. 871101) AB_2832879 (BioLegend Cat. No. 871102)
Antigen Details
- Structure
- Human EIF2AK2 has 2 isoforms. Isoform 1 is an 551 amino acid protein with predicted and observed molecular mass of ~62 kD; Isoform 2 is an 510 amino acid protein with predicted and observed molecular mass of ~57 kD
- Distribution
-
Tissue Distribution: Ubiquitously expressed
Cellular Distribution: Nucleus, Cytosol - Function
- Innate immune response against viral infection, central to stress signals
- Interaction
- Forms homodimer, interacts with DNAJC3, STRBP, ADAR
- Ligand/Receptor
- dsRNA
- Cell Type
- Astrocytes, Endothelial cells
- Biology Area
- Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Cell Death, Neurodegeneration, Neuroinflammation, Neuroscience, Signal Transduction
- Molecular Family
- APP/β-Amyloid, Innate Immune Signaling, Protein Kinases/Phosphatase
- Antigen References
-
- Chesnokova E, et al. 2017. Int J Mol Sci. 18(10).
- Bou-Nader C, et al. 2019. RNA. 25(5):539.
- Dzananovic E, et al. 2018. Biotechnol Genet Eng Rev. 34(1):33.
- Gene ID
- 5610 View all products for this Gene ID 54287 View all products for this Gene ID
- UniProt
- View information about EIF2AK2 on UniProt.org
