Product Details
- Verified Reactivity
- Human, Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Synthetic peptide corresponding to human Src phosphorylated at tyrosine 419
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
ICC, ICFC - Verified - Recommended Usage
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 1.0 ?g/mL.?For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/mL is recommended.?For intracellular flow cytometry using our True-Phos? Perm Buffer in Cell Suspensions Protocol, the suggested use of this reagent is ≤ 0.5 ?g per million cells in 100 ?L volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
This clone has not been tested for IP.
This clone may recognize other Src family members.
This clone was ICC tested on PFA-fixed cells using both methanol and Triton X-100 permeabilization methods. Both permeabilazation methods were compatible with staining. Triton X-100 permeabilization produces stronger staining.
During ICC product development testing, this clone produced strong nuclear staining in a small subpopulation (<1%) of both unstimulated and stimulated cells. The source of this staining is not clear but we do not believe it is Lck-dependent.
This clone was developed using a synthetic peptide corresponding to human Src phosphorylated at tyrosine 419, which displays high sequence homology with the region surrounding Lck tyrosine 394. To confirm specificity to Lck Phospho (Tyr394), the cell line J.Cam1.6 was used. This cell line is a clonal derivative of Jurkat cells that harbors a truncated LCK allele with defective protein expression (Straus, et al. 1992. Cell. 70:585).- Product Citations
-
- RRID
- AB_2814639 (BioLegend Cat. No. 933101) AB_2814640 (BioLegend Cat. No. 933102)
Antigen Details
- Structure
- Lck is a 509 amino acid protein with a predicted molecular weight of 58 kD
- Distribution
-
Plasma-membrane associated/T cells
- Function
- Protein Tyrosine Kinase
- Cell Type
- T cells
- Biology Area
- Cell Biology, Signal Transduction
- Molecular Family
- Protein Kinases/Phosphatase
- Antigen References
-
- Phillipsen L, et al. 2017. Sci Signal. 10:eaaf4736.
- Moogk D, et al. 2016. J Immunol. 197:644.
- Klammt C, et al. 2015. Nat Immunol. 16:961.
- Cancer Genome Atlas Network. 2015. Cell. 161:1681.
- Casas J, et al. 2014. Nat Commun. 5:5624
- McNeill L, et al. 2007. Immunnity. 27:425.
- Lefebvre DC, et al. 2003. Biochim Biophys Acta. 1650:40.
- Kabouridis PS. et al. 2003. Biochem J. 371:907.
- Abraham N, et al. 1990. Mol. Cell. Biol. 10:5197.
- Gene ID
- 3932 View all products for this Gene ID
- UniProt
- View information about Lck Phospho Tyr394 on UniProt.org
