Product Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Synthetic peptide corresponding to human EGFR surrounding Tyrosine 869 (Tyrosine 845 if signal peptide is excluded)
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
ICC, IP, ICFC - Verified - Recommended Usage
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 1.0 ?g per ml. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/ml is recommended.? For immunoprecipitation, the suggested use of this reagent is 2.5 ?g per ml.?For flow cytometric staining, the suggested use of this reagent is ≤ 0.125 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
This clone was tested for ICC using PFA-fixed A431 cells permabilized with methanol or Triton X-100. While both permeabilization methods were compatible with the antibody, Triton X-100 enabled superior staining.
This clone is predicted to recognize only isoforms I and V of EGFR due to the absence of the immunizing epitope in the other isoforms.- RRID
- AB_2810671 (BioLegend Cat. No. 617501) AB_2810672 (BioLegend Cat. No. 617502)
Antigen Details
- Structure
- EGFR is a 1,210 amino acid protein with a predicted molecular weight of 134 kD; the processed form is 1,186 amino acids in length.
- Distribution
-
Plasma membrane/Ubiquitously expressed
- Function
- EGFR signaling, cell signaling
- Ligand/Receptor
- EGF, TGFA/TGF-alpha, AREG, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF
- Biology Area
- Cell Biology, Signal Transduction
- Molecular Family
- Phospho-Proteins, Protein Kinases/Phosphatase
- Antigen References
-
- Hunter T and Cooper JA. 1981. Cell. 24:741.
- Gill GN and Lazar CS. 1981. Nature. 293:305.
- Reynolds FH, et al. 1981. Nature. 292:259
- Gene ID
- 1956 View all products for this Gene ID
- UniProt
- View information about EGFR on UniProt.org
