Product Details
- Verified Reactivity
- Human, Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Synthetic peptide corresponding to human S6 phosphorylated at serine 244
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
ICC - Verified - Recommended Usage
Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.1 - 1.0 ?g per ml.?For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
This clone is predicted to react with rat S6 when phosphorylated at serine 244 due to complete sequence homology between the immunizing sequence and the rat S6 ortholog.
- RRID
- AB_2820211 (BioLegend Cat. No. 933701) AB_2820212 (BioLegend Cat. No. 933702)
Antigen Details
- Structure
- RPS6 is a 249 amino acid protein with a predicted molecular weight of 28.6 kD.
- Distribution
-
Cytosol, nucleus, ER/ubiquitous expression
- Function
- Regulation of protein translation
- Biology Area
- Cell Biology, Cell Proliferation and Viability, Protein Synthesis, Signal Transduction
- Molecular Family
- Organelle Markers, Phospho-Proteins
- Antigen References
-
- Jefferies HB, et al. 1997. EMBO J. 16:3693.
- Ruvinsky I, et al. 2005. Genes. Dev. 18
- Schumacher AM, et al. 2006. Biochemistry. 45:13614
- Roux PP, et al. 2007. J. Biol. Chem. 282:14056.
- Stevens C, et al. 2009. J. Biol. Chem. 284:334.
- Schlafli P, et al. 2011. FEBS J. 278:1757.
- Gene ID
- 6194 View all products for this Gene ID
- UniProt
- View information about RPS6 Phospho Ser244 on UniProt.org
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