Product Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- KLH conjugated synthetic peptide corresponding to amino acids 428–454 of human complement C5 protein
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
IHC-P - Verified - Recommended Usage
Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 2.0 - 10 ?g/mL.?For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 2.0 - 10 ?g/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
This antibody predominantly detects C5b in human cell line and human plasma. At high concentration (between 2 and 10 ?g/mL), the full-length C5 is also detected at ~190 kD in human cell lines, but not in human plasma.
- RRID
- AB_2820182 (BioLegend Cat. No. 869901) AB_2820183 (BioLegend Cat. No. 869902)
Antigen Details
- Structure
- C5b is a 655 amino acid protein with predicted and observed molecular mass of ~ 75 kD.
- Distribution
-
Tissue Distribution: Predominantly in liver and plasma. Also expressed in the brain.
Cellular Distribution: Plasma membrane and extracellular - Function
- Complement C5b generation and assembly of MAC facilitates the lysis and destruction of pathogens.
- Interaction
- Interacts with C5a chain
- Ligand/Receptor
- Staphylococus aureus protein SSL5, binds C6 (transiently)
- Cell Type
- Astrocytes, Mature Neurons, Microglia
- Biology Area
- Cell Biology, Complement, Neurodegeneration, Neuroinflammation, Neuroscience
- Molecular Family
- Innate Immune Signaling
- Antigen References
-
- Vlaicu SI, et al. 2019. Front Immunol. 10:1054
- Fishelson Z, et al. 2019. Front Immunol. 10:752
- Bergseth G, et al. 2013. Mol Immunol. 56(3):232
- Gene ID
- 727 View all products for this Gene ID
- UniProt
- View information about Complement C5b on UniProt.org
