Product Details
- Verified Reactivity
- Human, Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Recombinant human ENO1 protein
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
KO/KD-WB, ICC, IP - Verified - Recommended Usage
Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.05 - 1.0??g/mL. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/mL is recommended. For immunoprecipitation, the suggested use of this reagent is 2.5??g/test. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
When tested for ICC using 4%-PFA fixed HeLa cells permeabilized with methanol or Triton X-100, high non-specific staining in the nucleus was observed. We therefore do not recommend these fix-perm methods for this clone.
- RRID
- AB_2892513 (BioLegend Cat. No. 945501) AB_2892513 (BioLegend Cat. No. 945502)
Antigen Details
- Structure
- ENO1 is a 434 amino acid protein with a predicted molecular weight of 47 kD.
- Distribution
-
Cytosolic/Ubiquitously expressed
- Function
- Glycolytic enzyme
- Biology Area
- Cell Biology
- Antigen References
-
- Capello M, et al. 2011. FEBS J. 278:1064-74
- Capello M, et al. 2016. Oncotarget. 7:5598-5612
- Cappello P, et al. 2017. Front Biosci (Landmark Ed). 22:944-959
- Díaz-Ramos A, et al. 2012. J Biomed Biotechnol. 2012:156795
- Didiasova M, et al. 2019. Front Cell Dev Biol. 7:61
- Isgrò MA, et al. 2015. Adv Exp Med Biol. 867:125-43.
- Liberti MV and Locasale JW. 2016. Trends Biochem Sci. 41:211-218.
- Gene ID
- 2023 View all products for this Gene ID
- UniProt
- View information about ENO1 on UniProt.org
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