Product Details
- Verified Reactivity
- Human, Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Synthetic peptide corresponding to human PDPK1 phosphorylated at serine 241
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
WB-KO/KD - Validated - Recommended Usage
Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 1.0??g/mL. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-A19011A was tested for ICC using 4% PFA-fixed HeLa cells permeabilized with either methanol or Triton X-100. While staining was observed, the signal was PDPK1-independent. We therefore do not recommend the clone for this application.
-A19011A is predicted to recognize the residue corresponding to Ser241 in multiple PDPK1 isoforms due to the presence of the immunizing sequence in each isoform.
-A19011A is predicted to recognize rat PDPK1 phosphorylated at Ser244 due to complete sequence homology between the immunizing peptide and the rat PDPK1 ortholog.
-A19011A may cross-react with PSK1-α when phosphorylated at Ser181 due to partial sequence homology with the immunizing peptide- RRID
- AB_2888741 (BioLegend Cat. No. 939601) AB_2888741 (BioLegend Cat. No. 939602)
Antigen Details
- Structure
- PDPK1 is a 556 amino acid protein with a predicted molecular weight of 63 kD. Four other PDPK1 isoforms have been described, with predicted molecular weights of 48, 51, 58, and 60 kD.
- Distribution
-
Ubiquitously expressed/cytosolic
- Function
- Serine-threonine protein kinase/cell signaling
- Biology Area
- Cell Biology
- Molecular Family
- Protein Kinases/Phosphatase
- Antigen References
- 1. Le Good JA, et al. 1998. Science. 281:2042.2. Casamayor A, et al. 1999. Biochem J. 342 Pt2):287.3. Dong LQ, et al. 2000. Proc. Natl. Acad. Sci. 97:5089.4. Hinton HJ, et al. 2004. Nat. Immunol. 5:539.5. Nagasima K, et al. 2005. J. Biol. Chem. 286:6433
- Gene ID
- 5170 View all products for this Gene ID
- UniProt
- View information about PDPK on UniProt.org
