Product Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Synthetic peptide corresponding to human STAT4 phosphorylated at tyrosine 693
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 647 under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
ICFC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Phos? Perm Buffer in Cell Suspensions Protocol. For flow cytometric staining, the suggested use of this reagent is 5 ?L per million cells in 100 ?L staining volume or 5 ?L per 100 ?L of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor? 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.
Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.
View full statement regarding label licenses- Excitation Laser
- Red Laser (633 nm)
- Application Notes
This antibody is predicted to recognize mouse STAT4 phosphorylated at tyrosine 694 and rat STAT4 phosphorylated at tyrosine 693 due to complete sequence homology between the immunizing peptide and the respective murine STAT4 orthologs.
Antigen Details
- Structure
- STAT4 is a 748 amino acid protein with predicted molecular weight of 86 kD.
- Distribution
-
Hematopoietic cells/Nucleocytoplasmic localization
- Function
- Transcription factor, cell signaling
- Biology Area
- Cell Biology, Signal Transduction, Transcription Factors
- Antigen References
-
Trinchieri G, et al. 2003. Nat. Rev. Immunol. 3:133.
Remmers EF, et al. 2007. N. Engl J. Med. 357:977.
Mukasa R, et al. 2010. Immunity. 32:616
Rapp M, et al. 2017. Sci. Immunol. 2:3296. - Gene ID
- 6775 View all products for this Gene ID
- UniProt
- View information about STAT4 Phospho on UniProt.org
