Product Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Recombinant mouse CX3CL1 (Fractalkine)
- Formulation
- 0.2 ?m filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is < 0.01 EU/?g of the protein (< 0.001 ng/?g of the protein) as determined by the LAL test.
- Preparation
- The Ultra-LEAF? (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF? solution contains no preservative; handle under aseptic conditions.
- Application
-
Neut - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by neutralization of the chemotaxis induced by recombinant mouse CX3CL1 (Fractalkine) (2 ?g /mL) on mouse Baf3-mCX3CR1 transfectant chemoattractant cells. ND50 range: 2-10 ug/mL. It is recommended that the reagent be titrated for optimal performance for each application.
- RRID
- AB_2894524 (BioLegend Cat. No. 938903) AB_2894524 (BioLegend Cat. No. 938904)
Antigen Details
- Structure
- Chemokine
- Distribution
-
Microglia, endothelial cells, fibroblasts, neuronal cells, hepatocytes, smooth muscle cells, monocytes, adipocytes, and human astrocytes stimulated by TNF-α and IFN-γ. CX3CL1 is also expressed in macrophages, fibroblasts, endothelial, and dendritic cells in RA synovial tissue
- Function
- CX3CL1 has potent chemoattractant activity for T cells, monocytes, and natural killer cells, and the expression of CX3CL1 in activated endothelial cells promotes strong adhesion of those leukocytes. CXC3L1 is induced in endothelial cells by proinflammatory cytokines (TNF-α, IFN-γ, IL-1β) and LPS.
- Interaction
- Monocytic cells, smooth muscle cells, T cells, B lymphocytes, NK cells, microglia, and astrocytes
- Ligand/Receptor
- CX3CR1
- Cell Type
- B cells, Endothelial cells, Microglia
- Biology Area
- Cell Biology, Neuroscience, Signal Transduction
- Molecular Family
- Cytokines/Chemokines
- Antigen References
-
- Bazan JF, et al. 1997. Nature. 385:640.
- Hundhausen C, et al. 2003. Blood. 102:1186.
- Sato E, et al. 2006. Ann Rheum Dis. 65:1257.
- Hundhausen C, et al. 2007 J Immunol. 178:8064.
- Jones B, et al. 2011. Front Immunol. 2:82.
- Hamann I, et al. 2011. Immunology. 133:62.
- Shah R, et al. 2011. Diabetes. 60:1512.
- Gene ID
- 20312 View all products for this Gene ID
- UniProt
- View information about CX3CL1 on UniProt.org
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