Product Details
- Verified Reactivity
- Human, Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Synthetic peptide conjugated to KLH
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
IHC-P, ICC - Verified - Recommended Usage
Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.125 - 0.5??g/mL.?For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 1.0 - 10.0??g/mL is suggested.?For immunocytochemistry, a concentration range of 2.5 - 5.0?μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
For ICC, we recommend 4% PFA fixation followed by permeabilization with ice cold methanol, or fixation/permeabilization with ice cold methanol alone. We do not recommend 4% PFA fixation followed by permeabilization with 0.5% Triton-X.
- RRID
- AB_2924609 (BioLegend Cat. No. 604751) AB_2924609 (BioLegend Cat. No. 604752)
Antigen Details
- Structure
- ATP1A1 can be processed by alternative splicing into 4 different isoforms; isoform 1 is a 1023 aa protein with a predicted molecular weight of 113kD.
- Distribution
-
ATP1A1 is widely expressed in the plasma membrane of most tissues.
- Function
- ATP1A1 is an ATPase involved in maintaining the balance of Na+ and K+ across of the plasma membrane.
- Biology Area
- Cell Biology, COVID-19
- Molecular Family
- Enzymes and Regulators, Ion Channels
- Antigen References
-
- Takase S, et al. 2017. Sci Rep. 7:2002.
- Wu IC, et al. Oncotarget. 2016 Dec 20;7(51):85244-85258
- Schlingmann KP, et al. 2018. Am J Hum Genet. 103:808-816.
- Zacherl S, et al. 2015. J Biol Chem. 290:3654-65.
- Azizan EA, et al. 2013. Nat Genet. 45:1055-60.
- Gene ID
- 476 View all products for this Gene ID
- UniProt
- View information about ATP1A1 on UniProt.org
