Product Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Synthetic peptide corresponding to human cdc2 phosphorylated at threonine 14
- Formulation
- Phosphate buffer, 0.5% BSA, 0.09% NaN3
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.25 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
ICC, ICFC - Verified - Recommended Usage
Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested dilution of this reagent is 1:4000. For immunocytochemistry, a dilution of 1:50?is recommended. For?intracellular flow cytometric staining, the suggested dilution of this reagent is 1:100 per million cells in 100 ?L volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
This clone is predicted to react with mouse cdc2 when phosphorylated at threonine 14 due to complete sequence homology between immunizing peptide and the cdc2 mouse orthologue.
This clone was tested for ICC using lambda protein phosphatase analysis and HeLa cells fixed with methanol or 4% PFA followed by permeabilization with either methanol or Triton X-100. Only PFA fixation followed by methanol permeabilization produced phospho-specific staining of cdc2.- RRID
- AB_2904458 (BioLegend Cat. No. 947401) AB_2904458 (BioLegend Cat. No. 947402)
Antigen Details
- Structure
- cdc2 is a 297 amino acid protein with a predicted molecular weight of 34 kD.
- Distribution
-
Ubiquitously expressed/ Mitochondria, nucleus, cytoskeleton, cytoplasm
- Function
- Serine/threonine-protein kinase/ Cell Cycle
- Interaction
- Cyclin B1, Cyclin A, PKMYT1 (MYT1)
- Biology Area
- Cell Biology, Cell Cycle/DNA Replication, Signal Transduction
- Molecular Family
- Protein Kinases/Phosphatase
- Antigen References
-
- Chow J, et al. 2011. Mol. Cell Biol. 31:1478-91.
- Diril M, et al. 2012. PNAS. 109:3826-31.
- Haneke K, et al. 2020. J. Cell. Biol. 219: e201906147.
- Liao H, et al. 2017. Oncotarget. 8:90662-73.
- Prevo R, et al. 2018. Cell Cycle. 17:1513-23.
- Gene ID
- 983 View all products for this Gene ID
- UniProt
- View information about cdc2 on UniProt.org
