Product Details
- Verified Reactivity
- Human, Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Synthetic peptide corresponding to human LAT phosphorylated at Tyr 171
- Formulation
- Phosphate Buffer, 0.5% BSA, 0.09% NaN3
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.05 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
ICC, ICFC - Verified - Recommended Usage
Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested dilution of this reagent is 1000 - 1:5000. For immunocytochemistry, a dilution of 1:10 is recommended. For intracellular flow cytometric staining, the suggested dilution of this reagent is 1:20 per million cells in 100 ?L volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
When this clone is used in WB at a dilution lower than 1:1000 it may show non-specific bands at 50 kD and above.
This clone has been tested by ICC on H2O2 treated and untreated Jurkat, with three fix and permeabilization methods (100% methanol, 4% PFA plus methanol and 4% PFA plus Triton X-100). All three methods can be used for ICC staining, but the PFA plus Triton X-100 fixation/permeabilization method produced the best staining.
This clone has only been tested on True-PhosTM perm buffer (Cat. No. 425401) by ICFC- RRID
- AB_2904456 (BioLegend Cat. No. 946601) AB_2904456 (BioLegend Cat. No. 946602)
Antigen Details
- Structure
- LAT is a 262 amino acid protein with a predicted molecular weight of 36-38 kD.
- Distribution
-
Plasma membrane/T Cells
- Function
- Plasma membrane/T Cells
- Cell Type
- Mast cells, NK cells, T cells
- Biology Area
- Cell Biology, Immunology, Signal Transduction
- Molecular Family
- Phospho-Proteins
- Antigen References
-
- Balagopalan L. et al., 2010, Cold Spring Harb Perspect Biol., 2(8): a005512
- Balagopalan L. et al., 2020, PLoS One, 15(2): e0229036
- Lin J. and Weiss A., 2001, J Biol Chem., 276(31): 29588-95
- Paz et. al., 2001, Biochem J., 356, 461-71
- Zhang W. et al., 2000, J Biol Chem., 275(30): 23355-61
- Gene ID
- 27040 View all products for this Gene ID
- UniProt
- View information about LAT Phospho Tyr171 on UniProt.org
