Product Details
- Verified Reactivity
- Human, Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Synthetic peptide of human AMPK alpha phosphorylated at Thr172
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
ICC - Verified - Recommended Usage
Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.125?- 1.0??g/mL.?For immunocytochemistry, a concentration?of 5.0?μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
This clone was tested for ICC using serum-starved untreated HEK293 cells (negative control) and HEK293 cells treated with 5.0 ?M oligomycin for 30 minutes (positive control). Staining was expected to localize to the nucleus and cytoplasm. Three fix/perm methods were used: methanol fixation or 4% PFA fixation followed by permeabilization with either methanol or Triton X-100. Both PFA fixation followed by methanol permeabilization and PFA fixation followed by Triton X-100 permeabilization produced a strong signal with the expected localization. Methanol-only fixation only produced a faint signal.
- RRID
- AB_2904438 (BioLegend Cat. No. 600651) AB_2904438 (BioLegend Cat. No. 600652)
Antigen Details
- Structure
- AMPKα is a 552 amino acid protein with a predicted molecular weight of 63 kD.
- Distribution
-
Heart, Skeletal muscle, Kidney / Nucleus and cytoplasm
- Function
- Serine/threonine-protein kinase, transcription regulation, Wnt signaling, metabolism
- Ligand/Receptor
- ATP-binding, magnesium, Metal-binding, Nucelotide-binding
- Biology Area
- Cell Biology, Signal Transduction
- Molecular Family
- Phospho-Proteins, Protein Kinases/Phosphatase
- Antigen References
-
- Mihaylova MM, et al. 2011. Nat Cell Biol 13:1016-23.
- Stein SC, et al. 2000. Biochem J. 345:437-43.
- Thaiparambil JT, et al. 2012. Mol Cell Biol. 16:3203-17.
- Vara-Ciruelos D, et al. 2019. Open Biol. 9:190099.
- Willows R, et al. 2017. Biochem J. 474:3059-3073.
- Gene ID
- 5563 View all products for this Gene ID 5562 View all products for this Gene ID
- UniProt
- View information about AMPKalpha Phospho Thr172 on UniProt.org
