Product Details
- Verified Reactivity
- SARS-CoV-2
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- SARS-CoV-2 Nucleocapsid recombinant protein
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
Direct ELISA, ICFC - Verified - Recommended Usage
Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.125 - 1.0 ?g/mL. For Direct ELISA application, a concentration of 3.7 ng/mL is recommended.?For?intracellular flow cytometric staining, the suggested use of this reagent is ≤ 0.5??g per million cells in 100 ?L volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
SARS-CoV-2 is 90% homologous to SARS-CoV. Cross reactivity of anti-SARS-CoV-2 Nucleocapsid antibody (clone A20087H) with SARS-CoV has not been tested.
BioLegend offers two clones specific for SARS-CoV-2 nucleocapsid, clones A20087F and A20087H- Both clones have been verified for western blot, with A20087F showing a higher affinity for SARS-CoV-2 nucleocapsid for this application
- Both clones have been verified for direct ELISA and displayed similar EC50 values for this application
- A20087H has been verified for ICFC, while A20087F is not recommended for this application
- RRID
- AB_2892515 (BioLegend Cat. No. 946101) AB_2892515 (BioLegend Cat. No. 946102)
Antigen Details
- Structure
- SARS-CoV-2 is a 419 amino acid protein with an expected molecular weight of 46 kD
- Distribution
-
Viral nucleoprotein, host cell Golgi apparatus
- Function
- RNA binding, transcription regulation
- Antigen References
-
- Zeng W. et al. 2020. Biochem Biophys Res Commun 527(3):618-623.
- Yoshimoto FK. 2020. Protein J. 39(3):198-216.
- Cascarina SM. & Ross ED. 2020. The FACEB Journal. 34:9832-9842.
- Gene ID
- 43740575 View all products for this Gene ID
- UniProt
- View information about Viral Protein on UniProt.org
