Product Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Recombinant human VEGF-165
- Formulation
- 0.2 ?m filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is < 0.01 EU/?g of the protein (< 0.001 ng/?g of the protein) as determined by the LAL test.
- Preparation
- The Ultra-LEAF? (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF? solution contains no preservative; handle under aseptic conditions.
- Application
-
Blocking - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by blocking the binding of 6 ng/mL biotinylated recombinant human VEGF-165 to 1 ?g/mL immobilized recombinant human VEGFR1 (Cat. No. 555804). ND50 range: 4 – 16 ?g/mL. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
This antibody has not been tested for ICC.
- RRID
- AB_2922643 (BioLegend Cat. No. 668053) AB_2922643 (BioLegend Cat. No. 668054)
Antigen Details
- Structure
- Homodimer
- Distribution
-
Widely expressed
- Function
- VEGFA is a key player in vasculogenesis, the formation of blood vessels from progenitor cells, as well as angiogenesis. The expression of the VEGFA gene is upregulated via hypoxia, estrogen, and NF-κB pathways. In addition, VEGFA is upregulated by PDGF-BB, P1GF, TGFβ1, IGF1, FGFs, HGF, TNFα, and IL-1. VEGFA induces proliferation and cell migration in endothelial cells, and plays important roles during wound healing. Also, VEGFA regulates haematopoietic stem cell survival.VEGFA interacts with vascular endothelial cells and monocytes/macrophages, which express VEGFR1. This interaction induces proliferation of endothelial cells and stimulates migration of monocytes/macrophages. VEGFR2 is expressed in endothelial cells and VEGFR2-signaling is essential for the development of vascular systems in the embryo.
- Interaction
- Embryonic Stem Cells, Mesenchymal Stem Cells, Neural Stem Cells
- Ligand/Receptor
- VEGFA binds and activates two tyrosine kinase receptors, VEGFR1 (Flt-1), VEGFR2 (KDR/Flk-1)
- Cell Type
- Embryonic Stem Cells, Mesenchymal Stem Cells, Neural Stem Cells
- Biology Area
- Angiogenesis, Cell Biology, Neuroscience, Stem Cells, Synaptic Biology
- Molecular Family
- Cytokines/Chemokines, Growth Factors
- Antigen References
-
- Conn G, et al. 1990. Proc Natl Acad Sci U S A. 87:1323-7.
- Gerber H, et al. 2002. Nature. 417:954-8.
- Shibuya M. 2006. J Biochem Mol Biol. 39:469-78.
- Reddy K, et al. 2008. Angiogenesis. 11:257-67.
- Shibuya M. 2008. BMB Rep. 41:278-86.
- Monaghan-Benson E, et al. 2010. Am J Pathol. 177:2091-102.
- Koch S and Claesson-Welsh L. 2012. Cold Spring Harb Perspect Med. 2:a006502.
- Gene ID
- 7422 View all products for this Gene ID
- UniProt
- View information about VEGF-165 on UniProt.org
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