Product Details
- Verified Reactivity
- Human, Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Synthetic peptide of human AKT phosphorylated at Ser473
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
ICC, ICFC - Verified - Recommended Usage
Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 ?g/mL.?For immunocytochemistry, a concentration range of 2.5 - 5.0?μg/mL is recommended.?For flow cytometric staining, the suggested use of this reagent is ≤ 0.03 ?g per million cells in 100 ?L volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
This clone was tested by Western Blot using lysates prepared from HeLa cells untreated or treated with IGF-1, and NIH/3T3 cell untreated or treated with PDGF.
This clone is suitable for ICC in cells treated with PFA and permeabilized with either methanol or Triton X-100.- RRID
- AB_2924618 (BioLegend Cat. No. 606551) AB_2924618 (BioLegend Cat. No. 606552)
Antigen Details
- Structure
- AKT is a 480 amino acid protein with a predicted molecular weight of 56 kD
- Distribution
-
Ubiquitously expressed, translocates to the membrane upon activation
- Function
- Catalytically inactive multimeric complex. AKT is activated by phospholipid binding and activation loop phosphorylation at Thr308 by PDK1 and by phosphorylation within the carboxy terminus at Ser473 by PDK2.
- Interaction
- Interacts through pleckstrin homology domain with second messengers. Interacts with AKTIP, CDKN1B.
- Antigen References
-
- Jacinto E, et al. 2006. Cell. 127:125-37.
- Inoki K, et al. 2002. Nat Cell Biol. 4:648-57.
- Franke TF, et al. 1995. Cell. 81:727-36.
- Brunet A, et al. 1999. Cell. 96:857-68.
- Yang WL, et al. 2010. Cell Cycle. 9:487-97.
- Gene ID
- 207 View all products for this Gene ID
- UniProt
- View information about AKT Phospho Ser473 on UniProt.org
