Product Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Synthetic peptide from human RORγt
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
ICFC, ICC - Verified - Recommended Usage
Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 1.0 ?g/mL.?For immunocytochemistry, a concentration range of 2.5 - 5.0?μg/mL is recommended.?For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 ?g per million cells in 100 ?L volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
This antibody does not cross react with mouse RORγ or RORγt.
- RRID
- AB_2927967 (BioLegend Cat. No. 609051) AB_2927967 (BioLegend Cat. No. 609052)
Antigen Details
- Structure
- RORγt is a 497 amino acid protein with a predicted molecular weight of 56 kD.
- Distribution
-
Expressed on different subsets of T cells (mature and immature), on thymus and lymph nodes. Also, it has been found expressed on Treg cells in colon.
- Function
- Promotion of pro-inflammatory Th17 cells by downregulation of Fas ligand and IL2 which reduce apoptosis.
- Ligand/Receptor
- Ligand are cholesterol metabolites
- Cell Type
- Lymphocytes, Macrophages, NK cells, NKT cells, T cells, Th17, Tregs
- Biology Area
- Adaptive Immunity, Immuno-Oncology, Immunology, Innate Immunity, Transcription Factors
- Molecular Family
- Innate Immune Signaling
- Antigen References
-
- Castro G, et al. 2017. PloS One. 12: e0181868.
- Villey I, et al. 1999. Eur J Immunol. 29:4072-80.
- Lamb D, et al. 2021. Respir Res. 22:158.
- Gene ID
- 6097 View all products for this Gene ID
- UniProt
- View information about RORgammat on UniProt.org
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