Product Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Synthetic peptide corresponding to human NF-κB p65
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
IHC-P, ICC, ICFC - Verified - Recommended Usage
Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 0.50 ?g/mL.?For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 1.0 - 10.0 ?g/mL is suggested.?For immunocytochemistry, a concentration range of 1.25 - 5.0 μg/mL is recommended.?For flow cytometric staining, the suggested use of this reagent is ≤ 0.125 ?g per million cells in 100 ?L volume. It is recommended that the reagent be titrated for optimal performance for each application
- Application Notes
When testing by ICC, we recommend fixation with 4% PFA followed by permeabilization with Triton X-100 or ice-cold methanol.
Antigen Details
- Structure
- NF-κB p65 is a 551 amino acid protein with a predicted molecular weight of 60 kD.
- Distribution
-
Ubiquitously expressed/ Cytoplasm and Nucleus
- Function
- Transcription, RAS pathway, host-virus interaction, oncogene
- Interaction
- NF-kappa-B, TLE5, TLE1, TP53BP2, GSK3B, MAP3K14, HDAC3, AKIP1, FOXP3
- Biology Area
- Cell Biology, Immuno-Oncology, Immunology, Innate Immunity, Transcription Factors
- Molecular Family
- Phospho-Proteins
- Antigen References
-
- Giridharan S and Srinivasan M. 2018. J Inflamm Res. 11:407-419.
- Ren C, et al. 2021. Cell Death Differ. 29:381-392.
- Yu S, et al. 2020. FEBS Lett. 594:1368-1378.
- Gene ID
- NA
- UniProt
- View information about NF-kB p65 on UniProt.org
