Product Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Recombinant mouse IL-27
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with biotin under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
- Application
-
ELISA Detection - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by?ELISA assay. A concentration of 0.125 ?g/ml of the capture antibody was utilized to generate the example standard curve. It is recommended that the end-user titrate each lot of reagents for optimal performance.
- Application Notes
ELISA Detection: To measure mouse IL-27, this antibody can be used as a detection antibody in sandwich ELISA format and paired with the purified MM27-7B1 antibody (Cat. No. 516902) as the capture antibody. Recombinant Mouse IL-27 (ELISA Std.) (Cat. No. 577409) can be used as the protein standard.
Note: For measuring mouse IL-27 in serum, plasma or cell culture supernatant, LEGEND MAX? Mouse IL-27 Heterodimer ELISA Kit with Pre-coated Plates (Cat. No. 438707 & 438708) are specially developed and recommended.- RRID
- AB_2563423 (BioLegend Cat. No. 525903) AB_2563424 (BioLegend Cat. No. 525904)
Antigen Details
- Structure
- A heterodimeric cytokine consisting of EBV-induced gene-3 (EBI3, an IL-12 p40-related protein) and p28 (a newly discovered IL-12 p35-related protein).
- Distribution
- Produced by antigen-presenting cells, including macrophages and dendritic cells.
- Function
- Drives rapid clonal expansion of na?ve CD4+ T cells, promotes Th1 polarization and IFN-?? production; plays a role in the anti-inflammatory and antitumor reponses, and has potential in autoimmune disease therapy.
- Interaction
- T cells and NK cells.
- Ligand/Receptor
- IL-27R (TCCR) (WSX-1).
- Cell Type
- Antigen-presenting cells, Macrophages, Dendritic cells, B cells
- Biology Area
- Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology, Signal Transduction
- Molecular Family
- Cytokines/Chemokines
- Antigen References
-
1. Salcedo R, et al. 2004. J. Immunol. 173:7170.
2. Owaki T, et al. 2005. J. Immunol. 175:2191.
3. Pflanz S, et al. 2002. Immunity 16:779.
4. Diveu C, et al. 2009. J. Immunol. 182:5748.
5. Morishima N, et al. 2005. J. Immunol. 175:1686.
6. Liu J, et al. 2007. J. Exp. Med. 204:141. - Gene ID
- 246779 View all products for this Gene ID
- UniProt
- View information about IL-27 on UniProt.org
