Product Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- E. coli - expressed, recombinant human IL-15
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with biotin under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
- Application
-
ELISA Detection - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by ELISA assay. For use as an ELISA detection antibody, a concentration range of 0.5-2.0 ?g/ml is recommended. To obtain a linear standard curve, serial dilutions of IL-15 recombinant protein ranging from 500 to 7.8 pg/ml are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
The biotinylated BH1543 antibody is useful as a detection antibody for a sandwich ELISA assay, when used in conjunction with purified BH1509 (Cat. No. 515002) antibody as the capture antibody and recombinant human IL-15 (Cat. No. 570309) as the standard.
- RRID
- AB_2561320 (BioLegend Cat. No. 515103) AB_2561321 (BioLegend Cat. No. 515104)
Antigen Details
- Structure
- 13 kD single non-glycosylated polypeptide
- Distribution
- T cells, B cells, dendritic cells, monocytes, and macrophages
- Function
- IL-15 stimulates the proliferation of activated T cells, NK cells, and B cells, inducing immunoglobulin synthesis by B cells stimulated by anti-IgM or CD40 ligand. In addition, IL-15 promotes the development of dendritic cells, activates human neutrophils, and induces the production of proinflammatory cytokines from macrophages.
- Interaction
- T lymphocytes, NK cells
- Ligand/Receptor
- IL-15Rα, IL-15Rβ and IL-15γc
- Cell Type
- T cells, B cells, Dendritic cells, Monocytes, Macrophages
- Biology Area
- Immunology, Innate Immunity
- Molecular Family
- Cytokines/Chemokines
- Antigen References
-
1. Grabstein K, et al. 1994. Science 264:965.
2. Ma A, et al. 2006. Annu. Rev. Immunol. 24:657.
3. Meresse B, et al. 2004. Immunity 21:357.
4. Armitage RJ, et al. 1995. J. Immunol. 154:483.
5. Pulendran B, et al. 2004. Eur. J. Immunol. 34:66.
6. Bouchard A, et al. 2004. J. Leuko. Biol. 75:893.
7. Ratthe C, et al. 2004. J. Leuko. Biol. 76:162.
8. Feng T, et al. 2008. Cell Immunol. 5:189.
9. Rubinstein MP, et al. 2006. P. Natl. Acad. Sci. USA 103:9166. - Gene ID
- 3600 View all products for this Gene ID
- UniProt
- View information about IL-15 on UniProt.org
