Product Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- E. coli - expressed, recombinant mouse IL-1α
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
- Application
-
ELISA Detection, ELISPOT Detection - Quality tested
WB, IP - Reported in the literature, not verified in house - Recommended Usage
Each lot of this antibody is quality control tested by ELISA assay. For use as an ELISA detection antibody, a concentration range of 0.05 - 0.2 ?g/ml is recommended. To obtain a linear standard curve, serial dilutions of mouse IL-1α recobinant protein ranging from 250 to 4 pg/ml are recommended for each ELISA plate. For Western Blotting applications, it is suggested to use 5 ?g antibody per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
ELISA Detection: The biotinylated BL1a-89 antibody is useful as a detection antibody for a sandwich ELISA when used in conjunction with purified BL1a-72 antibody (Cat. No. 512402) or purified ALF-161 antibody (Cat. Nos. 503202 & 503206) as the capture antibody and recombinant mouse IL-1a (Cat. No. 580309) as the standard.
- RRID
- AB_2295879 (BioLegend Cat. No. 512503) AB_2124220 (BioLegend Cat. No. 512504)
Antigen Details
- Structure
- Cytokine; 17.4 kD (Mammalian)
- Bioactivity
- Stimulates T cells, B cells, proliferation/activation of NK cells, fibroblasts, thymocytes, glioblastoma cells, astroglia, microglia; monocyte transition from IL-1β to IL-1α when matured to macrophages
- Cell Sources
- Monocytes, tissue macrophages, Langerhan cells, dendritic cells, T lymphocytes, B lymphocytes, natural killer (NK) cells, large granular lymphocytes (LGL), vascular endothelium, smooth muscle, fibroblasts, thymic epithelia, astrocytes, microglia, glioma,
- Cell Targets
- B cells, T cells, monocytes, fibroblasts
- Receptors
- Type I IL-1R (CDw121a), Type II IL-1R (CDw121b)
- Biology Area
- Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
- Molecular Family
- Cytokines/Chemokines
- Antigen References
-
1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. Bomford R, et al. Eds. 1989. Interleukin-1 inflammation and disease. Elsevier New York.
3. Brazel D, et al. 1991. Biotechnol. Ther. 2:241.
4. Dinarello C. 1996. Blood 87:2095. - Regulation
- Upregulated by TNF-α, IFN-α, IFN-β, IFN-γ, bacterial endotoxins, viruses, mitogens, antigens; downregulated by IL-6, lipoproteins, lipids, and α2-macroglobulin
- Gene ID
- 16175 View all products for this Gene ID
- UniProt
- View information about IL-1alpha on UniProt.org
