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Alexa Fluor® 488 anti-mouse CD206 (MMR) Antibo

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Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant mouse CD206 (MMR)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 488 under optimal conditions.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
FC, IHC-F　- Verified
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 ?g per million cells in 100 ?L?volume. For immunohistochemistry on frozen tissue sections, a concentration range of 2.5 - 5.0 ?g/mL?is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor? 488 has a maximum emission of 519 nm when it is excited at 488 nm.


Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Blue Laser (488 nm)
Application Notes

Clone C068C2 recognizes a region similar to clone MR5D3, based on the ability of the clones to block each other. Additional reported applications (for the relevant formats) include: spatial biology (IBEX)4,5.

Application References

(PubMed link indicates BioLegend citation)
  1. Keller J, et al. 2012. Biochem Biophys Res Commun. 417:217. PubMed
  2. Ito H, et al. 2012. J Am Soc Nephrol. 23:1797. PubMed
  3. Yang X, et al. 2015. PNAS. 112:2900. PubMed
  4. Radtke　AJ,　et al.　2020.　Proc Natl Acad Sci U S A.　117:33455-65. (SB)　PubMed
  5. Radtke　AJ,　et al.　2022.　Nat Protoc.　17:378-401. (SB)　PubMed
Product Citations
  1. Divakaruni AS et al. 2018. Cell metabolism. 28(3):490-503 . PubMed
  2. Arora H, et al. 2019. Immunity. 50:418. PubMed
  3. Orillion A, et al. 2018. Clin Cancer Res. 24:6383. PubMed
  4. Song J, et al. 2021. J Am Heart Assoc. 10:e017329. PubMed
  5. Weiss–Sadan T, et al. 2019. Cell Physiol Biochem. 53:550. PubMed
  6. Hreha TN, et al. 2020. Front Immunol. 1.597916667. PubMed
  7. Coppo M, et al. 2016. Nat Commun. 7:12254. PubMed
  8. Elbaz M, et al. 2015. Mol Oncol. 9: 906-919. PubMed
  9. Xu DQ, et al. 2021. Acta Pharmacol Sin. 42:1080. PubMed
  10. Xiong A, et al. 2022. EBioMedicine. 83:104239. PubMed
  11. Nasser MW, et al. 2015. Cancer Res . 75: 974-985. PubMed
  12. AR P, et al. 2016. Circ Res. 118: 400-409. PubMed
  13. Palathingal Bava E, et al. 2022. JCI Insight. 7:. PubMed
  14. Song L, et al. 2020. Am J Physiol Endocrinol Metab. 318:E1004. PubMed
  15. Hu D, et al. 2020. Am J Physiol Heart Circ Physiol. 318:H1525. PubMed
  16. Wu H, et al. 2019. Autophagy. :1. PubMed
  17. Shook BA, et al. 2020. Cell Stem Cell. 26(6):880-895. PubMed
  18. Jiang W, et al. 2021. J Cell Physiol. 236:7711. PubMed
  19. Lynch T, et al. 2017. J Mol Cell Cardiol. 102:83-93. PubMed
  20. Fields L, et al. 2021. Mol Ther. 29:2554. PubMed
  21. Choi EW, et al. 2020. Sci Rep. 10:12001. PubMed
  22. Song J, et al. 2021. Am J Physiol Heart Circ Physiol. 320:H323. PubMed
  23. Yang X, et al. 2015. Proc Natl Acad Sci U S A. 112:2900. PubMed
  24. Luck H, et al. 2019. Nat Commun. 10:3650. PubMed
  25. Ahmad F, et al. 2021. J Am Heart Assoc. 10:e017524. PubMed
  26. Tu W, et al. 2021. Cell Death Dis. 12:882. PubMed
  27. Lee SH, et al. 2022. Nat Commun. 13:5461. PubMed
  28. Calderon B, et al. 2015. J Exp Med. 212: 1497-1512. PubMed
  29. Alhudaithi SS, et al. 2020. Mol Pharm. 17:4691. PubMed
  30. Babazadeh S, et al. 2021. Cell Mol Biol Lett. 26:30. PubMed
  31. Molgora M, et al. 2020. Cell. 182:886. PubMed
  32. Zhang F, et al. 2021. J Immunol Res. 2021:5521051. PubMed
  33. Tang PC, et al. 2022. Adv Sci (Weinh). 9:e2101235. PubMed
  34. Mu?oz M, et al. 2022. ACS Nano. . PubMed
  35. Xiao M, et al. 2022. Mol Oncol. 16:1026. PubMed
  36. Barnette DN, et al. 2018. JCI Insight. 3. PubMed
  37. Yang F, et al. 2021. Nat Commun. 12:3424. PubMed
RRID
AB_10933252 (BioLegend Cat. No. 141709) AB_10900445 (BioLegend Cat. No. 141710)

Antigen Details

Structure
Type I transmembrane protein, 175 kD, C-type lectin superfamily
Distribution

Macrophages, dendritic cells, Langerhans cells, liver endothelial cells

Function
Pathogen recognition, endocytosis and phagocytosis, antigen presentation
Ligand/Receptor
Antigen containing mannose, fucose, or an N-acetyl glucosamine
Cell Type
Dendritic cells, Endothelial cells, Langerhans cells, Macrophages
Biology Area
Cell Biology, Immunology, Innate Immunity, Signal Transduction
Molecular Family
CD Molecules
Antigen References

1. Wileman TE, et al. 1986. P. Natl. Acad. Sci. USA 83:2501.
2. Apostolopoulos V, et al. 2001. Curr. Mol. Med. 1:469.
3. Burgdorf S, et al. 2006. J. Immunol. 176:6770.
4. McKenzie EJ, et al. 2007. J. Immunol. 178:4975.

Gene ID
17533 View all products for this Gene ID
UniProt
View information about CD206 on UniProt.org
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