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Purified anti-mouse CD206 (MMR) Antibody

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Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant mouse CD206 (MMR)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ICFC - Quality tested
FC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone C068C2 recognizes a region similar to clone MR5D3, based on the ability of the clones to block each other. Additional reported applications (for the relevant formats) include: spatial biology (IBEX)4,5.

Application References

(PubMed link indicates BioLegend citation)
  1. Keller J, et al. 2012. Biochem Biophys Res Commun. 417:217. PubMed
  2. Ito H, et al. 2012. J Am Soc Nephrol. 23:1797. PubMed
  3. Yang X, et al. 2015. PNAS. 112:2900. PubMed
  4. Radtke　AJ,　et al.　2020.　Proc Natl Acad Sci U S A.　117:33455-65. (SB)　PubMed
  5. Radtke　AJ,　et al.　2022.　Nat Protoc.　17:378-401. (SB)　PubMed
Product Citations
  1. Khan KA, et al. 2020. NPJ Breast Cancer. 6:29. PubMed
  2. Loyher PL, et al. 2018. J Exp Med. 215:2536. PubMed
  3. Wang W, et al. 2018. Cancer Cell. 34:757. PubMed
  4. Dasgupta D, et al. 2020. Gastroenterology. 159:1487. PubMed
  5. Zappasodi R, et al. 2021. Nature. 591:652. PubMed
  6. Hilligan KL, et al. 2020. Nat Commun. 11:5637. PubMed
  7. Nolin JD, et al. 2017. JCI Insight. 2:e94929. PubMed
  8. Khan KA, et al. 2020. NPJ Breast Cancer. 6:29. PubMed
  9. Bisht K, et al. 2021. Nat Commun. 12:5289. PubMed
  10. Sun KT, et al. 2018. Front Physiol. 1.06875. PubMed
  11. Hu G, et al. 2021. Nat Commun. 12:773. PubMed
  12. Enders M, et al. 2020. J Immunol. 204:87. PubMed
  13. Tian Z, et al. 2022. Front Integr Neurosci. 15:742377. PubMed
  14. Dong Y, et al. 2020. Front Immunol. 11:29. PubMed
  15. Blriot C, et al. 2021. Immunity. :. PubMed
  16. Gubin MM, et al. 2018. Cell. 175:1014. PubMed
  17. Campesato LF, et al. 2020. Nat Commun. 3.24375. PubMed
  18. Yang X, et al. 2015. Proc Natl Acad Sci U S A. 112:2900. PubMed
  19. Kim D, et al. 2019. Immune Netw. 19:e32. PubMed
  20. Scortegagna M, et al. 2020. Nat Commun. 11:99. PubMed
  21. Wang G, et al. 2019. Front Immunol. 1.380555556. PubMed
  22. Pushalkar S, et al. 2018. Cancer Discov. 0.613194444. PubMed
  23. Kikuchi K, et al. 2018. J Immunol. 201:635. PubMed
RRID
AB_10900263 (BioLegend Cat. No. 141701) AB_10900233 (BioLegend Cat. No. 141702)

Antigen Details

Structure
Type I transmembrane protein, 175 kD, C-type lectin superfamily
Distribution

Macrophages, dendritic cells, Langerhans cells, liver endothelial cells

Function
Pathogen recognition, endocytosis and phagocytosis, antigen presentation
Ligand/Receptor
Antigen containing mannose, fucose, or an N-acetyl glucosamine
Cell Type
Dendritic cells, Endothelial cells, Langerhans cells, Macrophages
Biology Area
Cell Biology, Immunology, Innate Immunity, Signal Transduction
Molecular Family
CD Molecules
Antigen References

1. Wileman TE, et al. 1986. P. Natl. Acad. Sci. USA 83:2501.
2. Apostolopoulos V, et al. 2001. Curr. Mol. Med. 1:469.
3. Burgdorf S, et al. 2006. J. Immunol. 176:6770.
4. McKenzie EJ, et al. 2007. J. Immunol. 178:4975.

Gene ID
17533 View all products for this Gene ID
UniProt
View information about CD206 on UniProt.org
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