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APC anti-mouse Ly-6A/E (Sca-1) Antibody

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Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
mouse pre-T cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: complement-mediated cytoxicity1,　immunohistochemical staining of frozen sections2 and Bouin-fixed, paraffin-embedded samples4, immunofluorescence microscopy4, and immunoprecipitation3.

The two Sca-1 recognizing clones D7 and E13-161.7 have been shown to bind distinct epitopes due to the inability of D7 to block the binding of E13-161.7.7

　

Application References

(PubMed link indicates BioLegend citation)
  1. Aihara Y, et al. 1986. Eur. J. Immunol. 16:1391. (CMCD)
  2. Spangrude GJ, et al. 1988. J. Immunol. 141:3697. (IHC)
  3. van de Rijin M, et al. 1989. P. Natl. Acad. Sci. USA 86:4634. (IP)
  4. van Bragt MPA, et al. 2005. Biol. Reprod. 73:634. (IHC, IF)
  5. Rosas M, et al. 2010. J. Leukoc. Biol. 88:169. PubMed
  6. Felthause O, et al. 2010. Neurosci Lett. 471:179. PubMed
  7. Bamezai A and Rock KL. 1995. Proc. Natl. Acad. Sci. USA 92:4294.
Product Citations
  1. Almada AE, et al. 2021. Cell Reports. 34(4):108656. PubMed
  2. Xie T, et al. 2021. Sci Adv. 7: . PubMed
  3. Karra L, et al. 2020. Oncogene. 6920:39. PubMed
  4. Bultmann–Mellin I, et al. 2017. Am J Physiol Lung Cell Mol Physiol. 313:L687. PubMed
  5. Papafragkos I, et al. 2022. Front Immunol. 13:889075. PubMed
  6. Britschgi A, et al. 2017. Nature. 541:541-545. PubMed
  7. Masschelein E, et al. 2020. Skelet Muscle. 10:21. PubMed
  8. Ogawa-Wong A, et al. 2022. Metabolites. 12:. PubMed
  9. Nishina T, et al. 2021. Nat Commun. 12:2281. PubMed
  10. Lopes C, et al. 2016. PLoS One. 11: 0162927. PubMed
  11. Simon M, et al. 2019. Cell Metab. 29:871. PubMed
  12. JI B, et al. 2016. Cell Death Differ. 23:759-75. PubMed
  13. Hulsmans M et al. 2017. Cell. 169(3):510-522 . PubMed
  14. Huang D, et al. 2020. Nat Commun. 4.520833333. PubMed
  15. Majumdar D, et al. 2021. Curr Protoc. 1:e216. PubMed
  16. Sakai H, et al. 2022. iScience. 25:103928. PubMed
  17. Celik H, et al. 2018. Cancer Cell. 34:741. PubMed
  18. Carmody C et al. 2019. Endocrinology. 160(5):1205-1222 . PubMed
  19. Siddique SM, et al. 2019. Sci Rep. 9:13977. PubMed
  20. Wang X, et al. 2018. Nat Commun. 9:551. PubMed
  21. An X, et al. 2020. Thyroid. . PubMed
RRID
AB_756196 (BioLegend Cat. No. 122511) AB_756197 (BioLegend Cat. No. 122512)

Antigen Details

Structure
Ly-6 multigene family, 18 kD
Distribution
Hematopoietic stem cells, activated T cells and B cells, subset of resting B cells and T cells
Function
Regulates B and T cell responses
Cell Type
Hematopoietic stem and progenitors, T cells, B cells, Mesenchymal Stem Cells
Biology Area
Immunology, Stem Cells
Antigen References

1. Rock KL, et al. 1989. Immunol. Rev. 111:195.
2. Morrison SJ, et al. 1994. Immunity 1:661.
3. Spangrude GJ, et al. 1988. J. Immunol. 141:3697.
4. Malek T, et al. 1986. J. Exp. Med. 164:709.

Gene ID
110454 View all products for this Gene ID
UniProt
View information about Ly-6A/E on UniProt.org
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