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Pacific Blue™ anti-mouse Ly-6A/E (Sca-1) Ant

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Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
mouse pre-T cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with Pacific Blue? under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The Ly-6A/E antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The suggested use of this reagent is ≤ 1.0 ?g per 106 cells in 100 ?l volume. It is highly recommended that the reagent be titrated for optimal performance for each application.

* Pacific Blue? has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue? conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.


Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Violet Laser (405 nm)
Application Notes

Additional reported applications (for the relevant formats) include: complement-mediated cytoxicity1,　immunohistochemical staining of frozen sections2 and Bouin-fixed, paraffin-embedded samples4, immunofluorescence microscopy4, and immunoprecipitation3.

The two Sca-1 recognizing clones D7 and E13-161.7 have been shown to bind distinct epitopes due to the inability of D7 to block the binding of E13-161.7.7

　

Application References

(PubMed link indicates BioLegend citation)
  1. Aihara Y, et al. 1986. Eur. J. Immunol. 16:1391. (CMCD)
  2. Spangrude GJ, et al. 1988. J. Immunol. 141:3697. (IHC)
  3. van de Rijin M, et al. 1989. P. Natl. Acad. Sci. USA 86:4634. (IP)
  4. van Bragt MPA, et al. 2005. Biol. Reprod. 73:634. (IHC, IF)
  5. Rosas M, et al. 2010. J. Leukoc. Biol. 88:169. PubMed
  6. Felthause O, et al. 2010. Neurosci Lett. 471:179. PubMed
  7. Bamezai A and Rock KL. 1995. Proc. Natl. Acad. Sci. USA 92:4294.
  8. Hu W, et al. 2015 Blood. 125:2206. PubMed
Product Citations
  1. Lawson H, et al. 2021. Stem Cell Reports. 16:2784. PubMed
  2. Hu W, et al. 2015. Blood. 125:2206. PubMed
  3. Barrett N, et al. 2016. Cell Rep. 16: 1039-1054. PubMed
  4. Symeonidou V, et al. 2021. Cell Rep. 37:109900. PubMed
  5. Paris J et al. 2019. Cell Stem Cell. 25(1):137-148 . PubMed
  6. Fossati V, et al. 2010. J Immunol. 184:1251. PubMed
  7. Karra L, et al. 2020. Oncogene. 6920:39. PubMed
  8. Wong J, et al. 2015. Elife. 3: 07839. PubMed
  9. Liu Y, et al. 2021. Haematologica. Online ahead of print. PubMed
  10. Grassinger J, et al. 2009. Blood. 114:49:00. PubMed
  11. Nguyen DTT, et al. 2020. Nat Commun. 11:2026. PubMed
  12. Eisele AS, et al. 2022. Elife. 11:. PubMed
  13. Lopez DA, et al. 2022. Cell Rep. 41:111677. PubMed
  14. Sala D, et al. 2019. Nat Commun. 10:1796. PubMed
  15. Courtney AH et al. 2017. Molecular cell. 67(3):498-511 . PubMed
  16. Yankova E, et al. 2021. Nature. 593:597. PubMed
  17. Booth CAG, et al. 2018. Cancer Cell. 33:274. PubMed
  18. Oben K, et al. 2017. PLoS One. 10.1371/journal.pone.0169767. PubMed
  19. Cheng Y, et al. 2019. Cell Rep. 28:1703. PubMed
  20. Zhang H, et al. 2019. Mol Cell. 76:110. PubMed
  21. Tzelepis K, et al. 2018. Nat Commun. 9:5378. PubMed
  22. Gerdes P, et al. 2022. Nat Commun. 13:7470. PubMed
  23. Ortega-Molina A, et al. 2021. Cell Reports. 36(2):109372. PubMed
  24. Yue F, et al. 2022. Cell Rep. 38:110267. PubMed
  25. Lee SG, et al. 2020. J Nucl Med. . PubMed
  26. Spevak CC, et al. 2020. Cell Stem Cell. 26(5):755-765. PubMed
  27. S?wen P et al. 2018. eLife. 7 pii: e41258. PubMed
  28. Park SM et al. 2018. Cell stem cell. 24(1):153-165 . PubMed
  29. Gozdecka M, et al. 2018. Nat Genet. 50:883. PubMed
  30. Ellis S, et al. 2011. Blood. 118:1516. PubMed
  31. Jee D et al. 2018. Molecular cell. 69(2):265-278 . PubMed
RRID
AB_2250602 (BioLegend Cat. No. 122519) AB_2143237 (BioLegend Cat. No. 122520)

Antigen Details

Structure
Ly-6 multigene family, 18 kD
Distribution
Hematopoietic stem cells, activated T cells and B cells, subset of resting B cells and T cells
Function
Regulates B and T cell responses
Cell Type
Hematopoietic stem and progenitors, T cells, B cells, Mesenchymal Stem Cells
Biology Area
Immunology, Stem Cells
Antigen References

1. Rock KL, et al. 1989. Immunol. Rev. 111:195.
2. Morrison SJ, et al. 1994. Immunity 1:661.
3. Spangrude GJ, et al. 1988. J. Immunol. 141:3697.
4. Malek T, et al. 1986. J. Exp. Med. 164:709.

Gene ID
110454 View all products for this Gene ID
UniProt
View information about Ly-6A/E on UniProt.org
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