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PE/Cyanine7 anti-mouse Ly-6A/E (Sca-1) Antibody

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Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
mouse pre-T cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 ?g per 106 cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: complement-mediated cytoxicity1,　immunohistochemical staining of frozen sections2 and Bouin-fixed, paraffin-embedded samples4, immunofluorescence microscopy4, and immunoprecipitation3.

The two Sca-1 recognizing clones D7 and E13-161.7 have been shown to bind distinct epitopes due to the inability of D7 to block the binding of E13-161.7.7

　

Additional Product Notes
BioLegend is in the process of converting the name PE/Cy7 to PE/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our PE/Cyanine7 products. Please contact Technical Service if you have any questions.
Application References

(PubMed link indicates BioLegend citation)
  1. Aihara Y, et al. 1986. Eur. J. Immunol. 16:1391. (CMCD)
  2. Spangrude GJ, et al. 1988. J. Immunol. 141:3697. (IHC)
  3. van de Rijin M, et al. 1989. P. Natl. Acad. Sci. USA 86:4634. (IP)
  4. van Bragt MPA, et al. 2005. Biol. Reprod. 73:634. (IHC, IF)
  5. Rosas M, et al. 2010. J. Leukoc. Biol. 88:169. PubMed
  6. Felthause O, et al. 2010. Neurosci Lett. 471:179. PubMed
  7. Bamezai A and Rock KL. 1995. Proc. Natl. Acad. Sci. USA 92:4294.
  8. Hara T, et al. 2012. J. Immunol. 189:1577. PubMed
Product Citations
  1. Chung Y, et al. 2014. J Vis Exp. 89: 51660. PubMed
  2. Mitroulis I et al. 2018. Cell. 172(1-2):147-161 . PubMed
  3. Yamamoto R et al. 2018. Cell stem cell. 22(4):600-607 . PubMed
  4. Guo B, et al. 2018. Nat Med. 24:360. PubMed
  5. Kobayashi H, et al. 2020. STAR Protoc. 1:100078. PubMed
  6. Hara T, et al. 2012. J Immunol. 189:1577. PubMed
  7. Lopez DA, et al. 2022. Cell Rep. 41:111677. PubMed
  8. Jia W, et al. 2021. Nat Commun. 12:2118. PubMed
  9. Chen X et al. 2017. Cell stem cell. 21(6):747-760 . PubMed
  10. Sá da Bandeira D, et al. 2022. Cell Rep. 40:111114. PubMed
  11. Bues J, et al. 2022. Nat Methods. 19:323. PubMed
  12. Nagai M, et al. 2020. Cell. 178(5):1072-1087.e14.. PubMed
  13. Cheng Y, et al. 2019. Cell Rep. 28:1703. PubMed
  14. Murakami K, et al. 2021. Cell Reports. 34(1):108579. PubMed
  15. Oguri Y, et al. 2020. Cell. 182(3):563-577.e20. PubMed
  16. Fournier M, Bijl C 2015. Stem Cells Dev. 24: 2413-2422. PubMed
  17. Sztwiertnia I, et al. 2020. PLoS One. 15:e0233789. PubMed
  18. Kobayashi H, et al. 2020. Cell Reports. 28(1):145-158.e9.. PubMed
  19. Kalafati L, et al. 2020. Cell. 183:771. PubMed
  20. Hoffmann J, et al. 2021. Nat Commun. 12:3964. PubMed
  21. Thürmer M, et al. 2022. Nat Commun. 13:2982. PubMed
  22. Lefkopoulos S, et al. 2020. Immunity. 53(5):934-951.e9. PubMed
  23. Muzumdar S, et al. 2019. Am J Pathol. 189:568. PubMed
  24. Rossnagl S, et al. 2016. PLoS Biol. 14: 1002562. PubMed
  25. Matsuzaki Y, et al. 2015. Biomed Rep. 1: 91 - 97. PubMed
  26. Luo H, et al. 2019. Cell Rep. 26:945. PubMed
RRID
AB_756198 (BioLegend Cat. No. 122513) AB_756199 (BioLegend Cat. No. 122514)

Antigen Details

Structure
Ly-6 multigene family, 18 kD
Distribution
Hematopoietic stem cells, activated T cells and B cells, subset of resting B cells and T cells
Function
Regulates B and T cell responses
Cell Type
Hematopoietic stem and progenitors, T cells, B cells, Mesenchymal Stem Cells
Biology Area
Immunology, Stem Cells
Antigen References

1. Rock KL, et al. 1989. Immunol. Rev. 111:195.
2. Morrison SJ, et al. 1994. Immunity 1:661.
3. Spangrude GJ, et al. 1988. J. Immunol. 141:3697.
4. Malek T, et al. 1986. J. Exp. Med. 164:709.

Gene ID
110454 View all products for this Gene ID
UniProt
View information about Ly-6A/E on UniProt.org
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