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Purified anti-mouse Ly-6A/E (Sca-1) Antibody

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Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
mouse pre-T cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IHC-F, IHC-P, IP - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 ?g per 106 cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: complement-mediated cytoxicity1,　immunohistochemical staining of frozen sections2 and Bouin-fixed, paraffin-embedded samples4, immunofluorescence microscopy4, and immunoprecipitation3.

The two Sca-1 recognizing clones D7 and E13-161.7 have been shown to bind distinct epitopes due to the inability of D7 to block the binding of E13-161.7.7

Application References

(PubMed link indicates BioLegend citation)
  1. Aihara Y, et al. 1986. Eur. J. Immunol. 16:1391. (CMCD)
  2. Spangrude GJ, et al. 1988. J. Immunol. 141:3697. (IHC)
  3. van de Rijin M, et al. 1989. P. Natl. Acad. Sci. USA 86:4634. (IP)
  4. Bamezai A and Rock KL. 1995. Proc. Natl. Acad. Sci. USA 92:4294.
  5. van Bragt MPA, et al. 2005. Biol. Reprod. 73:634. (IHC, IF)
  6. Brandi C, et al. 2009. Exp Eye Res. 89:209. PubMed
  7. Rosas M, et al. 2010. J. Leukoc. Biol. 88:169. PubMed
  8. Felthause O, et al. 2010. Neurosci Lett. 471:179. PubMed
  9. 　
Product Citations
  1. Schloss MJ, et al. 2022. Nat Immunol. 23:605. PubMed
  2. Chartrand K, et al. 2018. Front Immunol. 1.642361111. PubMed
  3. Goldsmith JR, et al. 2020. Nat Commun. 11:2591. PubMed
  4. Lee JH, et al. 2022. iScience. 25:104166. PubMed
  5. Yao L, et al. 2021. J Bone Miner Res. 36:1159. PubMed
  6. Gronowicz G, et al. 2017. Tissue Eng Part A. 23:1490. PubMed
  7. Felthaus O, et al. 2010. Neurosci Lett. 471:179. PubMed
  8. Brandl C, et al. 2009. Exp Eye Res. 89:209. PubMed
  9. Martin K,et al. 2017. J Immunol.. 10.4049/jimmunol.1601522. PubMed
  10. Shah K, et al. 2022. Nat Commun. 13:1827. PubMed
  11. Umemoto T, et al. 2017. EMBO J. 36:2390. PubMed
  12. Hsieh HY, et al. 2020. Cancer Sci. 111:2400. PubMed
  13. Nascimento DC, et al. 2017. Nat Commun. 8:14919. PubMed
  14. Helbling PM, et al. 2019. Cell Rep. 29:3313. PubMed
  15. Ishii H, et al. 2018. Nat Commun. 2.188194444. PubMed
  16. Rogers P, Driessnack M, and Schwartz E. 2017. PLoS One. 10.1371/journal.pone.0181985. PubMed
  17. Zhang C, et al. 2018. Cell Res. 28:323. PubMed
  18. Severe N et al. 2019. Cell Stem Cell. 25(4):570-583 . PubMed
RRID
AB_756186 (BioLegend Cat. No. 122501) AB_756187 (BioLegend Cat. No. 122502)

Antigen Details

Structure
Ly-6 multigene family, 18 kD
Distribution

Hematopoietic stem cells, activated T cells and B cells, subset of resting B cells and T cells

Function
Regulates B and T cell responses
Cell Type
B cells, Hematopoietic stem and progenitors, Mesenchymal Stem Cells, T cells
Biology Area
Immunology, Stem Cells
Antigen References

1. Rock KL, et al. 1989. Immunol. Rev. 111:195.
2. Morrison SJ, et al. 1994. Immunity 1:661.
3. Spangrude GJ, et al. 1988. J. Immunol. 141:3697.
4. Malek T, et al. 1986. J. Exp. Med. 164:709.

Gene ID
110454 View all products for this Gene ID
UniProt
View information about Ly-6A/E on UniProt.org
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