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PE anti-mouse GARP (LRRC32) Antibody

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Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse GARP (extracellular domain)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 ?g per million cells in 100 ?l volume.? It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Product Citations
  1. Geng S, et al. 2019. Sci Adv. 5:eaav2309. PubMed
  2. Timilshina M, et al. 2020. Cell Reports. 27(10):2948-2961.e7.. PubMed
RRID
AB_10983065 (BioLegend Cat. No. 142903) AB_10962944 (BioLegend Cat. No. 142904)

Antigen Details

Structure
80 kD transmembrane GARP glycoprotein with an extracellular region containing 20 leucine-rich repeats
Distribution
Activate Tregs, megakaryocytes, platelets
Ligand/Receptor
LAP (TGF-β1)
Cell Type
Tregs, Megakaryocytes, Platelets
Biology Area
Cell Biology, Immunology, Signal Transduction
Antigen References

1. Ollendorff V, et al. 1994. Cell. Growth Differ. 5:213.
2. Stockis J, et al. 2009. Eur. J. Immunol. 39:3315.
3. Wang R, et al. 2009. P. Natl. Acad. Sci. USA 106:13439.
4. Tran DQ, et al. 2009. P. Natl. Acad. Sci. USA 106:13445.

Gene ID
434215 View all products for this Gene ID
UniProt
View information about GARP on UniProt.org
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