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PE/Cyanine7 anti-human CD197 (CCR7) Antibody

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Product Details

Verified Reactivity
Human
Reported Reactivity
African Green, Baboon, Cynomolgus, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
CCR7-transfected cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Additional Product Notes

BioLegend is in the process of converting the name PE/Cy7 to PE/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our PE/Cyanine7 products. Please contact Technical Service if you have any questions.

Product Citations
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  5. Huang X, et al. 2021. Nat Nanotechnol. 16:214. PubMed
  6. Ishizaka A, et al. 2016. J Virol. 90: 5665 - 5676. PubMed
  7. Zeng W, et al. 2017. Front Immunol. 0.806944444. PubMed
  8. Mastelic-Gavillet B, et al. 2019. J Immunother Cancer. 7:257. PubMed
  9. Gao Y, et al. 2021. Oncogenesis. 10:62. PubMed
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  12. Schmidt J, et al. 2021. Cell Rep Med. 100194:2. PubMed
  13. Stephenson E, et al. 2021. Nat Med. 27:904. PubMed
  14. Zhang B, et al. 2021. Nat Biomed Eng. 5:1288. PubMed
  15. Kuebler P, et al. 2015. Proc Natl Acad Sci U S A. 112: 8379 - 8384. PubMed
  16. Mukhopadhyay M, et al. 2017. J Immunol. 10.4049/jimmunol.1700953. PubMed
  17. Thiel BA, et al. 2021. PLoS One. 16:e0249477. PubMed
  18. Nakasone H, et al. 2021. Commun Biol. 4:1177. PubMed
  19. Sefik E, et al. 2021. Nat Biotechnol. . PubMed
  20. Sureshchandra S, et al. 2022. Cell Rep. 39:110938. PubMed
  21. Gyurdieva A, et al. 2022. Nat Commun. 13:5296. PubMed
  22. Swanson E, et al. 2021. eLife. 10:00. PubMed
  23. Chellappa S, et al. 2016. J Leukoc Biol. 100: 5 - 16. PubMed
  24. Del Alcazar D, et al. 2019. Cell Rep. 28:3047. PubMed
  25. Pillet S, et al. 2016. Clin Immunol. 168:72-87. PubMed
  26. Rakshit S, et al. 2020. Cell Rep. 33:108451. PubMed
  27. Gannon PO, et al. 2020. Cytotherapy. 22(12):780-791. PubMed
  28. Yang B, et al. 2015. Cell Immunol. Available online 12 August 2015. PubMed
  29. Rydyznski Moderbacher C, et al. 2022. J Clin Invest. :. PubMed
  30. Savage AK, et al. 2021. iScience. 24(5):102404. PubMed
  31. Valle-Casuso JC, et al. 2019. Cell Metab. 29:611. PubMed
  32. Kim ST, et al. 2021. J Immunother Cancer. 9:. PubMed
  33. Genge PC, et al. 2021. STAR Protoc. 2:100900. PubMed
  34. Michaels K, et al. 2015. J Immunol. 194:3267. PubMed
  35. Kong XF, et al. 2018. Nat Immunol. 19:973. PubMed
  36. Tao L, et al. 2020. Cancers (Basel). 12:00. PubMed
  37. Mackroth M, et al. 2016. PLoS Pathog. 12:e1005909. PubMed
  38. Gannon P, et al. 2015. J Immunol. 195: 356 - 366. PubMed
  39. Franchini DM et al. 2019. Cell reports. 26(1):94-107 . PubMed
  40. Fu J, et al. 2021. J Clin Invest. 131:. PubMed
  41. Siedlik J, et al. 2017. J Immunol Methods. 10.4049/jimmunol.1700003. PubMed
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RRID
AB_11125576 (BioLegend Cat. No. 353225) AB_11126145 (BioLegend Cat. No. 353226)

Antigen Details

Structure
Chemokine receptor, G protein-coupled receptors (GPCR), seven transmembrane receptor.
Distribution

T cells, B cells, NK, dendritic cells.

Function
The chemokine receptor CCR7 plays a pivotal role in the homing of na?ve T cells and regulatory T cells to secondary lymphoid organs, and the migration of dendritic cells into afferent lymphatic vessels.
Ligand/Receptor
CCL19 and CCL21.
Cell Type
B cells, Dendritic cells, NK cells, T cells
Biology Area
Immunology
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors, GPCR
Antigen References

1. Yanagihara S,　et al.　1998.　J. Immunol.　161:3096.
2. Charo IF,　et al.　2006.　N. Engl. J. Med.　354:610.
3. Reif K,　et al.　2002.　Nature　416:94.
4. Nakata B,　et al.　2008.　Oncology　74:69.
5.　Brodie T.　et al. 2013.　Cytometry A.　6: 530-2.　PubMed
6. Graves A.J. et al. 2014. Cytometry A. 7: 576–9　PubMed
7. Moncunill G. et al. 2014. Cytometry A. 12: 995-8　PubMed

Gene ID
1236 View all products for this Gene ID
UniProt
View information about CD197 on UniProt.org
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