Product Details
- Verified Reactivity
- Human, Mouse, Rat, All species
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- KLH-conjugated His-tag peptide.
- Formulation
- 0.2 ?m filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.01 EU/?g of the protein (<0.001 ng/?g of the protein) as determined by the LAL test.
- Preparation
- The Ultra-LEAF? (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF? solution contains no preservative; handle under aseptic conditions.
- Application
-
WB - Quality tested
ICC, FC - Verified - Recommended Usage
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1:1000-1:10000 diluted (0.05 - 0.5 ?g/mL).?For immunocytochemistry, a concentration range of?0.1 - 1.0 ?g/mL (1:500 - 1:5000 dilution) is recommended.?For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 ?g per million cells in 100 ?L?volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
This antibody was raised against a linear 8X His tag. It reacts with proteins displaying repeats of 6 Histidines (His6) or more in either internal or C and N terminal regions. It is suitable for ICFC.
Clone J095G46 has been verified for immunocytochemistry (ICC).- RRID
- AB_2814310 (BioLegend Cat. No. 362615) AB_2814311 (BioLegend Cat. No. 362616)
Antigen Details
- Structure
- 6 repeat His amino acid
- Function
- Epitope tag
- Biology Area
- Cell Biology, Immunology
- Antigen References
-
- Zhao C, et al. 2010. Anal Biochem. 399:237
- Salichs E, et al. 2009. PLoS Genet. 5:e1000397
- Terpe K. 2003. Appl. Microbiol. Biotechnol. 60:523
- Lindner, P, et al. 1997. Biotechniques. 22:140
- Burritt JB, et al. 1995. J. Biol. Chem. 270:16974.
- Taylor RM, et al. 2007. Methods Mol. Biol. 412:429.
- Gene ID
- NA
- UniProt
- View information about His Tag on UniProt.org
