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Alexa Fluor® 647 anti-His Tag Antibody

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Product Details

Verified Reactivity
All species (6x His tag epitope)
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
KLH-conjugated His-tag peptide
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 647 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 0.2 - 0.5 μg/ml (Dilution?1:1000-1:2500) is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor? 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Burritt JB, et al. 1995. J. Biol. Chem. 270:16974.
  2. Taylor RM, et al. 2007. Methods Mol. Biol. 412:429.
Product Citations
  1. Yusuf B, et al. 2021. Sci Rep. 11:3614. PubMed
  2. Ellis GI, et al. 2022. STAR Protoc. 3:101784. PubMed
RRID
AB_2716153 (BioLegend Cat. No. 652513)

Antigen Details

Function
Recombinant poly-histidine tag detection
Biology Area
Cell Biology
Gene ID
NA
UniProt
View information about His Tag on UniProt.org
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